Jan. 1904] 
Cultures of Uredineae in 1903. 
u 
Five species of rusts were successfully grown, that had been 
studied with success before, and reported upon by the writer and 
in part by other invesigators. Mention of them here serves to 
confirm previous work, and in two cases to give additional knowl¬ 
edge regarding hosts. 
1. Puccinia Impatientis (Schw.) Arth.—Teleutosporic 
material of this species on Elymus Virginicus was obtained near 
Lafayette, Ind., and sown, May 13, on Impatiens aurea. On May 
18, an abundance of spermogonia appeared, and on May 26, an 
equal abundance of aecidia began to show. This result confirms 
the work of last year. 3 The locality from which the teleuto¬ 
sporic material was obtained for this season’s work is several 
miles from the one yielding material last year. 
2. Puccinia amphigena Diet.—Teleutosporic material was 
obtained by the writer on Ccilaniovilfa longifolia from the type 
locality at Chicago, Ill. A sowing was made on a mature leaf of 
Smilax hispida, May 23. Spermogonia appeared sparingly on 
May 29, but were not followed by aecidia. Another sowing was 
made on a partly grown leaf of the same host species, May 24, 
from which a great abundance of spermogonia began to appear 
on May 28, followed by an equal abundance of aecidia, June 
5. Some question has been raised regarding the correctness of 
last year’s work, 4 as the teleutosporic form is known from re¬ 
gions where it is thought that Smilax does not grow, but that 
the genuine Puccinia amphigena has its alternate form on Smilax 
can no longer be doubted. 
3. Puccinia Andropogonis Schw.—Teleutosporic material 
<on Andropogon scoparius, collected at Bloomington in southern 
Nebraska, was sent to Rev. J. M. Bates. It was sown on Pent- 
stemon hirsutus, May 19, giving rise to abundant spermogonia 
on May 25, followed by aecidia on June 4. Previous cultures 
have been made by Mr. William Stuart and the writer, 5 and by 
Prof. W. A. Kellerman. 5 There can be no question that this is 
■a wide-spread and common species in North America. 
4. Puccinia albiperidia Arth.—Teleutosporic material of 
this species on Carex gracillima was most opportunely sent from 
Racine, Wis M by Dr. J. J. Davis. Sowing was made April 21, on 
Ribcs Cynosbati , from which abundant spermogonia appeared on 
April 30, followed by great numbers of aecidia on May n. A 
sowing made at the same time on R. doridum gave no infection. 
Three days later a sowing made on R. aureum seemed to have 
made a slight growth, which, nevertheless, came to naught. A 
sowing on R. Uva-crispa , a cut branch being placed in a glass of 
3 Bot. Gaz. 35 : 18. 1903. 
4 Bot. Gaz. 35 :20. 1903. 
6 Bot. Gaz. ^9 :272. 1900. 
*Jour. Mycol. 9:10. 1903. 
