Jan. 1904] 
Cultures of Uredineae i?i 1903. 
17 
The collection on Car ex varia Muhl., made at Fair Oaks, 
Ind., March 22, 1903, is taken as the type, together with the re¬ 
sult of the culture on Solidago Canadensis L. obtained by a sow¬ 
ing made June 3, and matured July 1, 1903, at which date it was 
placed in the herbarium. I would tentatively refer here the collec¬ 
tions referred to above on C. gracillima, C. pubescens, C. vires- 
eens (?) and C. lanuginosa, leaving their exact status to be deter¬ 
mined later. 
The aecidium of this species does not appear to differ in 
any marked manner from that of Puccinia Caricis-Solidaginis 
Arth., although, perhaps, the spores are a trifle smaller. I have 
not, however, had opportunity of collecting it in the field, as the 
heavy spring rains flooded the type locality and prevented all 
subsequent development of the rust, so that in subsequent visits 
at different times during the season the most dilligent oearch 
failed to reveal any trace of it on either Solidago or Car ex. 
6. Aecidium pustulatum Curt.—In early April, 1902, an 
observation was made at Spirit Lake, Iowa, that proved very 
puzzling for a time. On an open prairie, that had been burned 
•over during the late fall, a small area showed Comandra pallida 
A. DC. with aecidia, and in contact with it Andropogon scoparius 
Michx. bearing uredo, of the characteristic thin-walled sort 
known to belong to the species with aecidium on Pentstemon. 
It was easy to find teleutospores on the grass leaves pressed into 
hollows, thus protected from the passing fire, and only in part 
germinated. These were collected and sown on Pentstemon 
hirsutus with no infection. A sowing was not made on Coman¬ 
dra, as no suitable growing plants were available. A similar 
observation was made again this year at Fair Oaks, Ind., and 
once more under circumstances that seemed to permit of no 
other inference but that the Comandra and Andropogon rusts 
were connected, highly improbable as it seemed. This time 
plants of Comandra umbellata (L.) Nutt, were secured. A sow¬ 
ing of teleutospores from Andropogon furcatus Muhl. was made 
on May 5, and spermogonia began to appear on May 16, but the 
host plant withered before time for aecidia to appear. A similar 
sowing was made on a more vigorous host, May 25, spermo¬ 
gonia appearing in great abundance May 30, and aecidia June 9. 
Another sowing of teleutospores from A. scoparius obtained in 
the same locality at Fair Oaks, was made on Comandra umbel¬ 
lata June 1, the first spermogonia appearing June 9, and aecidia 
June 19. Sowings of both sets of teleutospores were made twice 
on Pentstemon hirsutus, under the most favorable circumstances, 
with no infection. The conclusion is beyond all question, that 
a common rust on species of Andropogon, not readily distin¬ 
guishable from Puccinia Andropogonis Schw., has its aecidia on 
Comandra, being identical with Ae. pustulatum Curt. 
