Jan. 1906 ] Cultures of Uredineae in fpojy 
13 
another host, were made in 1903 on six other species of hosts 
with negative results. 5 
4. Puccinia Crandallii Pamm. •& Hume, on Festuca 
condnis, sent from Boulder, Colo., by Mr. E. Bethel, was sown 
on Dodecatheon Meadia, Hydrophyllum appendiculatum, Aqui- 
legia Canadensis, Anemone Canadensis, Thalictrum dioicum, 
Rudbeckia triloba, Gutierrezia Sarothrae, and Lonicera Japonica, 
with no infection. 
5. Uromyces graminicola Burr, on Panicum virgatum, 
sent from Red Cloud, Neb., by Rev. Bates, was sown on Pso- 
ralea Onobrychis, with no infection; while similar material sent 
by Mr. Bartholomew from Stockton, Kans., was sown on the 
same host and also on Cassia Chamaecrista, Polemonium reptans 
and Rudbeckia laciniata, all with no infection. 
6. Uromyces Junci (Schw.) Tul. on Junicus effusus, sent 
by Mr. Jackson from Ithaca, N. Y., was sown on Rudbeckia 
laciniata, R. triloba, and Falcata comosa, with no infection. 
What is believed to be the same species of rust, but on another 
host, was sown in 1902 on a species of Iris with negative results. 
The following species of rusts were successfully grown, and 
the data supplement that obtained from previous cultures of this 
series, or that published by other investigators obtained by means 
of similar cultures. The results in connection with Puccinia 
Pruni-spinosae, the plum rust, are especially interesting, being 
the first studies of the kind with American material. 
1. Melampsora Medusae Thuem.— Teleutosporic mate¬ 
rial obtained near Lafayette, Ind., on Populus deltoides was sown 
April 17 on Larix laricina and L. decidua, which resulted in 
abundance of spermogonia appearing April 25, and a greater 
abundance of aecidia May 1, upon both hosts. 6 
2. Gym NOSPORANGIUM JUNIPERI-VIRGINIANAE Schw. 
Three samples of teleutosporic material were used, one from the 
eastern states, and two from the central west. The test was 
designed to show whether any difference existed between the 
two regions in the power of the species to infect the cultivated 
apple; the apple orchards of Iowa and the central west generally 
being well known to be especially free from this rust. All the 
teleutosporic material was on Juniperus Virginiana, and to all 
appearances equally good. That sent from Durham, N. C., by 
Mr. Wolfe, was sown on a seedling apple out of doors April 27, 
and showed abundant spermogonia May 9, but did not develop 
5 See Jour. Myc. 10 : 10. 1904. 
“See Jour. Mycol. 10 : 13. 1904; and 11 : 52. 1905. 
