14 
Journal oj Mycology 
[Vol. 12 
aecidia, although conditions appeared favorable. Material from 
Washington, Iowa, sent by Rev. Echlin, was sown April 22, on 
a small plant of the York Imperial apple in the greenhouse, and 
showed abundant spermogonia on May 5; another sowing was 
made out of doors on a seedling apple April 27, and showed 
spermogonia on May 13, but further observation was prevented 
by insect depredation. A second lot of material was received 
from Ames, Iowa, sent by Mr. Buchanan, and was sown out of 
doors on seedling apple and Crataegus coccinea, and in the 
greenhouse on Amelanchier Botryapium. The sowing on apple 
gave abundant spermogonia, but failed to make further develop¬ 
ment, while on the other hosts there was no infection. So far as 
these tests go there appears to be no difference between the east¬ 
ern and western forms of the species in their power to infect the 
cultivated apple. Many cultures have been made showing the 
full life cycle of this rust (often called G. macropus) . A good 
summary of the subject, especially in reference to the question 
underlying the present work, was given a short time ago by 
Professor Pammel 7 of the Iowa State College. 
3. Puccinia Sambuci (Schw.) Arth. — Teleutosporic 
material of fine quality on Carex lupulina, collected in Noble 
county, near Beavor Dam, Ind., was sent by Mr. Whetzel. It 
was sown on Sambucus Canadensis May 8, giving rise to abun¬ 
dant spermogonia May 14, and aecidia May 25. 
4. Puccinia albiperidia Arth. — Teleutosporic material 
was gathered near Lafayette, Ind., on Carex tetanic a, and sown 
April 7 on Silphium perfoliatum, Polemonium reptans, Ambrosia 
trifida, Rudbeckia laciniata, Steironema ciliatum, and Ribes gra- 
cile. Only the last host showed infection, giving abundant sper¬ 
mogonia April 14, and aecidia April 27, the others remained 
entirely free from rust. 
A part of the teleutosporic material was sent to Dr. H. Kle- 
bahn, Hamburg, Germany, who sowed it on Ribes Uva-crispa, 
R. aureum and R. rubrum. No infection resulted on R. rubrum, 
but on R. Uva-crispa spermogonia showed May 8, and on R. 
aureum May 15. The further development, however, was slow, 
soon coming to naught in the first case, and persisting much 
longer, but with little advance in the second case. Dr. Klebahn 
in commenting upon his results says that the imperfect develop¬ 
ment of the infection on R. uva-crispa may be ascribed, in part 
at least, to the late sowing, for the teleutospores germinated 
freely. The host plants had already passed the most active period 
of growth, and the weather was too warm. His results are 
exactly in accord with our own under similar conditions. The 
7 Bull. Iowa Exper. Sta., No. 84:16-24. August, 1905. 
