20 
fouriial of Mycology 
[Vol. 12 
plum) and from Anemone ranunculoides on P. spinosa, produc¬ 
ing in each the characteristic uredospores of Puccinia Pruni- 
spinosae, the rust of plums and peaches. 
This work by Dr. Tranzschel suggested the following trials 
with American material. Aecidiospores from Hepatica acutiloba 
(Aecidium hepticatum Schw.) were sown on three small plants 
of Prunits serotina (wild black cherry), established in pots in the 
greenhouse, on Primus Americana (native plum), P. Cerasus 
(cultivated cherry), and Amygdalus Persica (peach), the last 
two seedlings. The sowings were made May I to 4, and in fifteen 
days afterward, uredospores appeared on P. serotina, but the 
other plants remained wholly free, watch being continued for a 
month and more. The successful sowings were as follows: 
May 1, Aecidiospores sown on P. serotina; May 16, uredospores; May 22, 
teleutospores. 
May 1, Aecidiospores sown on P. serotina; May 16, uredospores. 
May 2, Aecidiospores sown on P. serotina; May 17, uredospores. 
On May 23, a sowing of uredospores, which had been grown 
on P. serotina, was made on A. Persica, under seemingly most 
favorable conditions, but no infection took place, watch being 
kept for two months. 
From these results there can be no further question of the 
general identity of the American and European plum and cherry 
rusts, and their connection with the Aecidium punctatum. It is 
not possible to state what significance is to be attached to the 
failure to infect peach, plum and cultivated cherry with spores 
that readily infected the wild cherry. Careful search for two 
seasons in the vicinity of the diseased hepaticas, the fungus being 
perennial, has failed to detect any rust on plums, cherries or 
peaches, wild or cultivated, although growing in plenty; and 
furthermore Puccinia Pruni-spinosae has not yet been reported 
from Indiana, although said to occur in the adjoining state of 
Illinois on P. serotina, P. Virginiana, and P. Americana. 
20. Puccinia Xanthii Schw. — Teleutosporic material 
on Xanthium Canadense, gathered near Lafayette, Ind., on Nov. 
5, 1904, was sown on the second and third leaves of seedlings of 
the same species of host April 3. On April 8 small yellow dots 
began to show, which closely simulated spermogonia, but which 
microscopic examination by means of sections proved were only 
the very young teleutosporic sori. The yellow dots increased in 
size, appearing like small pimples, and finally broke through the 
epidermis, exposing the teleutospores April 21, eighteen days 
after inoculation. Another sowing was made April 13, on the 
cotyledons of the same species of host, and on the second leaves 
of Ambrosia triftda. The Xanthium seed-leaves showed yellow 
dots on April 22, and open sori May 1, also in eighteen days 
from sowing, but the Ambrosia leaves remained free. 
