Jan. 1906] 
Cultures of Uredineae in 1905 
23 
turing, than Puccinia elegans Schrot., reported only from Argen- 
tine, South America, an original specimen of which I have been 
able to examine through the courtesy of Dr. P. Hennings of the 
Botanical Garden, Berlin. P. transformans has not yet been 
reported from any locality in the United States. 
5. Puccinia Kuhniae Schw. — Teleutosporic material, 
gathered near Lafayette, Ind., Nov. 3, 1904, on Kuhnia eupatori- 
oides, was sown on the same host May 19. On May 28 spermo- 
gonia appeared sparingly, which were examined under the micro¬ 
scope in section, and these were followed June 5 by uredo in 
fair abundance. The species, therefore, belongs to the group 
of brachypuccinia. 
6. Puccinia canaliculata (Schw.) Lagerh. — An obser¬ 
vation in the field made in 1904 led to the present successful cul¬ 
tures. Aecidia were found in remarkable abundance on seedling 
Xanthium Canadense over an area 8 to 10 meters in diameter, 
beyond which the aecidium did not occur, although the hosts 
were equally plentiful and equally exposed. Later in the season, 
the middle of June, uredosori were found upon what appeared 
to be a seedling sedge extending over approximately the same 
area that had been occupied in the spring by the cocklebur cluster 
cups, and this was followed in August by teleutosori. Although 
the sedge did not fruit, yet it was not difficult to ascertain that 
it was a species of Cyperus, and the rust Puccinia canaliculata. 
On May 11 a sowing of aecidiospores, obtained from the 
locality mentioned, was made in the greenhouse on Cyperus 
esculentus, other species of the genus not being at hand, and on 
June 2 uredo were noticed, although they probably appeared ear¬ 
lier and were overlooked, being small and pale. Another sow¬ 
ing was made on the same species of host May 17, and uredo 
first noticed June 2. The plants did not grow well, and the 
infected leaves were removed for the herbarium before time 
enough had elapsed in which to develop telutosori. Cultures with 
teleutosporic material will doubtless confirm this association of 
the Xanthium and Cyperus rusts. 
7. Puccinia Eleochardis Arth.— Teleutosporic material 
on Eleocharis palustris was sent by Dr. Davis from Racine, Wis., 
and with it the information that from observations in the field 
he believed this to have its aecidial phase on Eupatorium. Act¬ 
ing upon this suggestion, a sowing was made May 5 on Eupa¬ 
torium perfoliatum, which gave rise to spermogonia May 13, and 
aecidia May 22. Another sowing on the same species of host May 
11, gave spermogonia May 20, and aecidia May 30. Both trials 
produced an abundance of aecidia, with all the characteristics of 
the common and widely distributed form on this host, and closely 
related species. 
