Journal of Mycology 
46 
[Vol. 12 
good host. Culture on Chenopodium hybridum failed; probably 
made too late. It has been made earlier this year. 
April 18th I brought down from Orleans Puccinia poculi- 
formis on Elymus canadensis to test on a hedge of Berberis vul¬ 
garis. It looked very strong, but when the aecidia appeared, I 
could find no more near the culture than fifty feet away, and but 
little more than the year before without culture. Nor could I find 
any such Puccinia growing within several blocks of the hedge, 
ithough abundant on Hordeum jubatum and other hosts a mile 
south. 
May 14th I made a culture of Puccinia amphigena, Calamo- 
vilfa longifolia, on the only plant of Smilax hispida found in three 
years, in this region. It grows a mile away from the grass, and, 
the year before, had no secidium on it. The winter before my 
experiment, it had been cut to the ground. The fresh shoots were 
therefore in fine condition for this late experiment. I had no 
chance to view it until June 10, when I found the whole plant 
covered with secidia. As the Puccinia grows in abundance miles 
away from any species of Smilax in Cherry Co. there can be no 
doubt that it has the same faculty as Puccinia poculiformis of 
living over without the first stage. The appearance of this rust 
at the late date of May 14 is specially interesting. I hope to test 
it much earlier this year. 
June 14, three miles west of Red Cloud, I found one plant of 
Oenothera biennis, about 15 inches high, with ripe secidia cover¬ 
ing the under side of the lowest leaves and unripe ones follow¬ 
ing the leaves as they developed to the very summit. As Aecidium 
Peckii comes in distinct sori, I saw that I had something new, 
and gathered all that was fit. Looking then for the clue, I found 
Carex Pennsylvania growing all around it and uredo in abund¬ 
ance within three feet, growing scarcer as you departed from 
the source, until at four feet there was none at all. The patch of 
grass land on which it grew had been burned over, the previous 
winter, so that I found No. Ill, though of course some of it had 
escaped. I collected a set of the uredo. June 20 I was in 
Sargent, Custer Co., and found the same rust on Oenothera 
biennis, Oe. sinuata and Carex Pennsylvania, in vacant town lots. 
Four miles out, I found it again, and the next day in Arcadia 
again. Dr. Bessey says he found it once in Iowa on Oe. biennis 
and Prof. Holway reports the same. Nov. 3, I was able to collect 
a set of III. on Carex Pennsylvania at Red Cloud, and since 
then have made collections of same at Sargent. The III. looks 
like a pale weak uredo, but Prof. Holway reports it as a good 
teleutosporic form and a new species. 
I have given these details, because it seems to me that the 
relationship between the two or three hosts is abundantly estab¬ 
lished. Nevertheless “to make assurance doubly sure,” I have 
