52 
Journal of Mycology 
[Vol. 8 
July 21, Aecidiospores from Euphorbia nutans sown on E. nutans; 
July 30, uredo. 
July 21, Aecidiospores from E. nutans sown on E. maculata; no 
infection. 
July 21, Uredospores from E. nutans sown on E. nutans; July 31, 
uredo. 
July 21, Uredospores from E. nutans sown on E. maculata; no 
infection. 
There are two ways in which the right species of plant may 
be selected on which to make a sowing of a hetercecious rust. 
One is to sow upon any species known to harbor an aecidium, 
hoping after making a smaller or larger number of tests to 
hit upon the right one. This method in my own work has led 
to very meagre results, so slight, in fact, as scarcely to be worth 
the trouble. During 1901 the following rusts were tried in 
this blind way, all being very common forms in this vicinity, 
only to ascertain that wrong plants were selected for the sow¬ 
ings or else the spores did not gain entrance possibly through 
defective treatment. 
Puccinia emaculata Schw. on Panicum capillare was sown 
twice on Onagra biennis, with no infection. 
Puccinia caricina DC. A form of this aggregate on Carex 
tetanica was sown on Erigeron annuus and Onagra biennis, with 
no infection. 
Puccinia atkinsoniana Diet, on Carex lurida. The name 
of this species was unknown at the time the cultures were made, 
but through the kindness of Professor Atkinson, it has since been 
compared with type material and its status definitely settled. 
It was sown on Aster cordifolius, Aster paniculatus, Solidago 
serotina, Xanthium canadense, Ambrosia trifida, Impatiens aurea 
and Ribes cynosbati, with no infection. 
Puccinia peckii (DeT.) Kellerm. on Carex trichocarpa. The 
names of both host and rust were unknown at the time the 
cultures were made. The host has since been found in fruit, 
and the rust has been successfully grown by Professor Keller- 
man and also the writer, as recorded in the preceding number 
of this Journal (8:20). Spores were sown on Aster cordifolius, 
Aster paniculatus, Solidago serotina, Solidago canadensis, Ribes 
cynosbati, Erigeron annuus, and Eupatorium perfoliatum, with no 
infection. 
Puccinia rubigo-vera DC. A form of this aggregate of 
Bromus ciliatus was sown on Hepatica acuta and Viola cucullata, 
with no infection. 
During 1901 the culture of seven species was successfully 
carried out. Three of these were in confirmation of previous 
work, as follows: 
Puccinia caricis (Schum.) Reb. April 25, teleutospores from 
Carex stricta were sown on Urtica gracilis; May 2, spermogonia 
appeared, and May 5, aecidia. 
