126 
M. J. Le Goc. 
the extremities of the ateral outgrowths minute conidia are formed. 
Besides I refer to his own work on this question (Brefeld, VII, p. 
73). Only I note that by supplying a richer nutritive solution, 
according to Brefeld, other branchings and branching systems are 
laid down as conidiophores. Moller insists on Brefeld’s statement 
and has also obtained a more or less abundant production of the 
characteristically hooked conidia. Unfortunately he gives no draw¬ 
ings to confirm this point. As already mentioned I obtained 
different results. I have not even succeeded in bringing out the 
germination of spores in water. As Brefeld states that often eight 
days or more are required before germination begins to become 
later on general, I left the spores often three weeks; but of conidia 
formation there was no trace to be observed. On the contrary, 
the spores became divided by partition walls and emitted germina¬ 
tion tubes; but in spite of every attempt, any further formation 
failed.” 
Like Holtermann I repeatedly tried to get results agreeing 
with those of Brefeld, but always failed in my attempts, at least as 
far as nutritive solutions were concerned. 
The spores were cultivated in hanging drops in sealed cells, 
and easily observed under the microscope. 
Experiments were carried out in strong, medium and weak 
light, and even in darkness. Under these conditions germination 
was never observed in hanging drops of water, but in Elder wood 
decoctions it occurred occasionally. 
However, if a drop of the decoction is laid at the bottom of the 
same cell instead of being suspended from the cover glass, germi¬ 
nation takes place regularly. The probable explanation of this 
anomally is that the spores being heavier than the liquid fall to the 
bottom, so that in the hanging-drop cultures the spores are only 
partly immersed and osmosis is not carried out in the normal 
manner. In tubes or watch glasses the spores germinate very 
readily in Elderwood decoction, and rarely in distilled water. Like 
Holtermann I found that the spores 'germinate on the third day, 
but if the spores had not germinated by this time further develop¬ 
ment was impossible even after allowing them an opportunity which 
lasted more than a fortnight. Invariably after some five days drops 
of glycogen were formed in any ungerminated spores which at a 
later stage became ruptured and emitted their contents (Fig. 3, b,c). 
Brefeld, on the contrary, observed spores often germinating after 
being immersed for a period lasting more than eight days (3, p. 72). 
