Franklin Kidd & Cyril West. 
metabolism resulting from deficiency in oxygen supply and 
accumulation of carbon dioxide (cf, Jodin, 3 ; and Mazd, 4), (2) that 
it is due to the leaching out of essential soluble food-reserves, (3) 
that it is due to a combination of (1) and (2). 
In the case of seeds soaked under the same conditions except 
as regards temperature, although no direct data are available, it 
would appear that at the lower temperatures there would be more 
available oxygen for the following reasons : (1) the greater solubility 
of oxygen at lower temperatures, and (2) the relatively greater 
decrease in the rate of respiration as compared with the rate of 
diffusion of oxygen at lower temperatures. Hence the question of 
oxygen supply must be eliminated in seeking to explain the 
differences in the effect of soaking seeds at higher and lower 
temperatures. 
We may assume that at lower temperatures the amount ot 
carbon dioxide in the water of soaking (other conditions being the 
same) will not be greater than at higher temperatures; there is 
evidence, however, that at low temperatures carbon dioxide is more 
active in producing inhibition and injury than at higher temperatures 
(Kidd, 5). 
In connection with the above experiments we obtained data as 
to the exosmosis or leaching out of soluble food-reserves which 
occurred at various temperatures, and the following tables (Tables 
111 and IV) give the results obtained. 
Table III. 
Effect of various temperatures on the exosmosis of soluble substances that 
occurs when Dwarf Runner Beans (Phaseolus vulgaris, var.J are soaked in 
excess of distilled water. 
Period of soaking : 3 days. Seeds soaked under 4 cms. of water. 25 seeds 
used in each experiment. 
Temp, of the water used 
10°C. 
20 a C 
25°C 
30° C 
Total weight of solid mat¬ 
ter leached out of 25 bean 
0-572 gins. 
0 528 gms. 
0 563 gms. 
1 270 gms. 
seeds during experiment 
Table IV. 
Effect of various temperatures on the exosmosis of soluble substances that 
occurs when Peas (Suttons “ Maincrop ”) are soaked in excess of distilled 
water. 
Period of soaking : 3 days. Seeds soaked under 4 cms. of water. 50 seeds 
used in each experiment. 
Temp, of the water used 
5°C 
10°C 
20°C 
30° C 
Total weight of solid mat¬ 
ter leached out of 50 pea 
0 278 gms. 
0-253 gms. 
0-220 gms. 
0-659 gms. 
seeds during experiment 
