3 l6 
A. E. Lechmere. 
in Ieucin solution with mycelium taken from the skin of fish, the 
shape of the sporocyst was that typical of Saprolegnia, the discharge 
of several was observed and seen to consist of motile zoospores, the 
swarming was very feeble and all spores came to rest quite near the 
sporocyst (Fig. 26). As very few spores were formed the position 
of each was carefully noted and the slide was kept under the 
microscope for observation. After five hours the spores were all 
found to have germinated directly in the same position (Fig. 29). 
No signs of any empty cases were found in the culture so that it 
was concluded that either the second motile stage had been sup¬ 
pressed, or that the zoospores had been discharged in the second 
motile stage—in the latter case they show the characteristic 
feature of the genus Pythiopsis. 
(7) . Achlya-type. In shape the actual sporocyst resembles 
that of Saprolegnia , but it differs in the manner in which its spores 
are discharged. Many cases were found where the spores collected 
into an irregular mass at the mouth of the sporocyst (Fig. 28), in 
which position encystment took place, and after the usual period of 
rest, the zoospores emerged from the cyst-wall, and entered upon 
the second motile stage, which is really the only “ motile” stage in 
this genus, as although the zoospores on discharge have cilia, they 
only swarm to the mouth of the sporocyst. Resemblance to the 
genus Adilya was also seen in the method of branching of the 
sporocyst (Figs. 9 and 16), and in the formation of new sporocysts as 
lateral branches from the base of the old sporocyst. These con¬ 
ditions were found in a twelve days’ old culture, on the skin of fish 
in water, where they seemed to be the normal method. The same 
conditions have been found from time to time on various hanging 
drop cultures. 
(8) . Dictyuchus-type. In a hanging drop culture in water 
taken from a nine days’ old culture on white of egg, the numerous 
resting sporocysts present on the mycelium commenced to develop 
into sporocysts. In by far the greater number of cases observed, 
the spores encysted within the sporocyst before its discharge, in 
spite of the fact that a tubular process was developed on the sporo¬ 
cyst before the spores encysted (Figs. 30, 31). The zoospores 
were observed leaving the cyst-walls and escaping through the 
tubular process, pushing before them the empty cases which were 
in the tube. The property of encysting within the sporocyst is 
characteristic of the genus Dictyudius. This condition was 
