18 Dorothea C. E. Marry at. 
structures highly typical of, and chiefly confined to, the Basidio- 
mycetes—set all doubts on this score at rest. On carefully teasing 
out small pieces of the mycelium, it was found that the majority of 
the spores appeared to be produced interstitially at some distance 
from one another along the length of the hyphae, after the manner 
of typical chlamydospores. Sometimes, however, single terminal 
spores might be observed at the ends of short branches (Plate I., 
Fig. 7). The spores were thick-walled and colourless, but were 
filled, as were also the hyphae, with numerous highly-refractive 
oil-granules. They varied in size from 12 X 8//, to 8 X 6ft. 
Since it was not easy to tease out the hyphae of the mycelium 
very satisfactorily and observe in detail how the spores were being 
produced, hanging drop cultures were made of them in (a) Water 
(/3) Gelatine and 10% Grape Extract. 
These both proved very successful; in the Water hanging drops, 
the spores began to germinate twenty-four hours after they were 
sown, sending out short, colourless germ-tubes (Plate I., Fig. 2). 
The following day these germ-tubes had increased considerably in 
length (Fig. 3), and on the third day had begun to branch (Fig. 4). 
By the sixth day interstitial spores or chlamydospores were produced 
in both the main hypha and its branches. Clamp-connections had 
also begun to appear (Fig. 5). On the seventh day, the interstitial 
spores had increased in number, and i't was interesting to note that 
in many cases a spore had been formed immediately beneath the 
original parent-spore, which, though still attached above, was by 
this time completely devoid of contents (Fig. 6). At this stage, the 
water-drops became fouled with Bacteria and it was difficult to 
make further observations. The Gelatine and Grape-extract drops 
showed an interesting set of stages in the development of the 
spores and could be kept for much longer than the water-cultures, 
but owing to the nature of the medium it was not so easy to follow 
the course of events under the microscope. 
The spores germinated well, but development seemed slower 
than in the water-cultures and a considerable quantity of repeatedly- 
branched mycelium was produced—in which the clamp-connections 
could be seen very beautifully—before any new spores were formed. 
The latter were not noticed until the tenth day, though later they 
appeared in considerable numbers. It was often easy to find single 
spores produced at the ends of short branches, as well as the 
typical interstitial arrangement; the former are interesting in con¬ 
nection with Brefeld’s observations, which will be referred to later. 
