>38 
T. G. Hill 
It remains to be demonstrated whether they are capable of 
raising their internal osmotic strength in proportion to the increase 
of the external salinity, which part of the investigation was carried 
on during April of this year. 
In order to show this, sods containing seedlings of Salicornia 
were taken from a pan which had been covered by the tide a few 
hours previously. The turf was placed in a dish of fresh water, 
which was changed periodically, so that the salinity was gradually 
reduced. From time to time a seedling was removed and its root- 
hairs tested with a 3-5 % solution of sodium chloride. Eventually 
nine consecutive seedlings showed a marked plasmolysis of their 
root-hairs. These were allowed to remain in a bath of saline of 
the stated strength (3’5%) and were examined next morning ; when 
it was found that, of those root-hairs which were favourable for 
observation, 81% showed no plasmolysis, and the remaining 19%, 
although slightly plasmolysed, were not affected to anything like 
the same degree as when they were first examined. At the first 
immersion in the 3'5% solution of sodium chloride, the protoplasm 
shrank away, not only from the top of the root-hair, but also from 
the sides (Fig. 21) ; at the second test, the appearance of the 
plasmolysis, in the cases where it occurred, was as illustrated in 
Fig. 22 
Fig. 22 
Fig. 21. 
In order that the natural conditions might be reproduced as 
nearly as possible, a portion of the soaked sod was bathed in a 
3-5% solution of salt, which was sufficiently strong to plasmolyse 
the sample seedlings examined. After the treatment, the result 
was found entirely to confirm the results of the preceding 
experiment. 
The following observations were then made on the hairs of 
a restricted area of the roots of a fresh batch of Salicornia, which 
had been previously treated with fresh water. Of these, rough 
charts were drawn in order that the same root-hairs could be kept 
