t94 Walter Stiles and Ingvar Jorgensen 
between the two sets of cultures is thus several times greater than 
the probable error, and there can be no doubt of its significance. 
The results thus agree with those obtained by Hall, Brenehley 
and Underwood with Barley. 
Buckwheat. 
Series 4. 
The recent experiments of Free (4) at Baltimore failed to show 
any improvement in the growth of water cultures of Buckwheat as 
a result of aeration. 
The seed used in this series was obtained from Messrs. Carter- 
The culture solution was composed as follows : 
Calcium nitrate (cryst.) ... 
Magnesium sulphate (cryst.) 
Potassium nitrate 
Sodium chloride ... 
Potassium dihydrogen phosphate 
Ferric chloride 
Tap water... 
F00 gm. 
0-25 „ 
0-25 „ 
0-06 „ 
0-25 „ 
004 „ 
1000-00 cc. 
Seeds sown in Sphagnum, July 5. 
Seedlings transferred to solutions, July 11. 
Solutions changed, July 21, August 13, 22. 
Cultures harvested, August 24. 
The leaf development was not followed in this series. The 
dry weights obtained after 50 days from the date of sowing are 
given below. They are given in full as they are instructive in com¬ 
parison with the numbers obtained in the previous series as showing 
the great effect of inherited factors in water culture experiments. 
The buckwheat seed was not a pure line, and in consequence 
variations due to inherited factors are much more prominent. 
Aerated. 
Plant 
Dry Weight in grams 
Shoot Root Whole Plant 
1 
1-977 
0-190 
2-167 
2 
0-680 
0-078 
0-758 
3 
1-256 
0161 
1-417 
4 
1-362 
0-128 
1-490 
5 
1-763 
0-157 
1-920 
6 
1-507 
0-163 
1-670 
7 
0-271 
0 044 
0-315 
8 
1-090 
0-202 
1-292 
9 
0-943 
0-090 
1033 
10 
1-775 
0-146 
1-921 
Mean 
1-398 + 0-120 
