Methods of Reproduction in the Saprotegnicecz. 169 
induce formation of gemmae. From these various cultures small 
pieces of the mycelium were teased off and mounted as hanging 
drop cultures to follow in detail the formation and discharge of the 
sporocysts and the formation of oocysts. 
Examination of the First Species. 
Species I. This species in general appearance resembled very 
closely the species described in the previous paper, the chief point 
of difference being the tendency to form oocysts at a very early 
stage. The oocysts were often formed soon after sporocyst 
formation had commenced, instead of occurring as is usually the 
case at a later stage, when the formation of sporocysts has been 
continued for some time. 
In this feature it shows a marked contrast to the former species, 
which could not be induced to form sexual organs under any 
conditions, but in all the other features it shows a very close 
resemblance. 
Typical life-history of Species I. The following is the account 
of the life-history of this species as shown by numerous hanging 
drop cultures and small pieces frequently teased off the cultures in 
the Petri dishes. 
The mycelium at first consists of slender coenocytic hyphae 
showing a tendency for the protoplasm to condense at the apex, 
causing swelling of the hyphae. Very soon growth is arrested by 
the formation of a terminal club-shaped sporocyst containing the 
zoospores arranged in several rows (p. 170, fig. 1). 
The zoospores are formed in the typical manner and are dis¬ 
charged in the motile state through a short terminal process (p. 170, 
fig. 2). After discharge, they swarm actively for a short time before 
coming to rest and encysting, in which state they rest for a period 
of eight to twenty-four hours. After the resting-period is completed 
the contents of the spore protrudes as a small bud and soon the 
whole contents is discharged through the small aperture in the 
spore wall, and then enters upon the second motile stage, leaving 
the empty spore case behind (p. 170, fig. 3). Numerous empty cases 
are seen in the hanging drop cultures. 
The same difference was noticed in the form of the two kinds 
of zoospores, as already noted (loc. cit., p. 313). 
After the discharge of the zoospores, a new sporocyst grows up 
inside the wall of the one first formed ; numerous cases showing 
