Methods of Reproduction in the Saprolegniacece. 179 
When once the sporocyst has been discharged there remains 
the question of the formation of the new sporocyst. It is typical 
of the genus Saprolegnia for this to take place by a simple upgrowth 
(durchwachsend) of the hypha within the discharged sporocyst. 
This process being repeated several times, a series of cases, one 
within the other, is produced, giving the appearance known as 
“ nested sporocysts.” 
This process, with various modifications, was certainly found 
to occur (p. 180, fig. 8), but at the same time a'second and entirely 
different process was noticed. 
It has been previously noted that the sporocysts were often of 
different lengths, and the method of formation of the new sporocyst 
seems to depend to some extent upon the length of the first formed 
sporocyst. 
The length of the sporocyst on a vigorous mycelium is usually 
eight to ten times the diameter, and, in the case of very long forms, 
twelve times or more. 
After observing the discharge of numerous sporocysts (p. 180, 
figs. 1 and 2), the cultures were watched in order to observe the up¬ 
growth of the new sporocyst, and, on re-examining the next day, they 
showed for the most part the appearance of a new sporocyst formed 
immediately below the first one (p. 180, fig. 3); this same appearance 
could be produced by upgrowth within a long sporocyst terminating 
half-way (p. 180, fig. 4). Closer attention was paid to this point, 
and cases were found where the first formed sporocyst was ready 
to discharge the zoospores, and immediately below it a part of the 
hypha had been delimited, containing dense granular protoplasm 
and giving exactly the appearance of an early stage in sporocyst 
formation (p. 180, fig. 5). 
After the discharge of the terminal sporocyst in such a case, 
the segment below was found soon to contain zoospores ready for 
discharge. 
The discharge of this new sporocyst is terminal, and the 
zoospores have to traverse the empty cyst in front, before reaching 
the exterior proper (p. 180, fig. 6). 
After the discharge of the second sporocyst, or during the 
formation of zoospores, a third sporocyst is formed, immediately 
below the second, in a similar manner (p. 180, figs. 6 and 7). 
The third sporocyst proceeds to form and discharge its 
zoospores, which now have to traverse two empty cases in front of 
them before attaining freedom. 
