Methods of Reproduction in the Saprolegniacece. 181 
As they are not very active on discharge, the zoospores from 
the third sporocyst often do not reach the exterior, but encyst 
within one of the empty cases; examples were also noted where 
they had germinated in this position. 
The greatest number of sporocysts found to have been formed 
in this way, and which have discharged motile zoospores, is three ; 
one case was noted where a fourth had been delimited, but no 
further development took place. 
From the great number of cases observed, especially in the 
young and vigorous cultures, this method seems to be of fairly 
general occurrence in this species. 
A second method of forming the new sporocyst was noted in 
cases where the first sporocyst was of the more usual club-shaped 
type instead of being, as in the first case, just described, of an 
elongated cylindrical form. 
Here the hypha was found to grow up inside the empty case in 
the usual manner (p. 180, fig. 8), but when this had been completely 
filled, the growth of the hypha still continued, so that a pro¬ 
truding bud was formed ; this bud increased in size until it had 
the appearance of a somewhat smaller sporocyst born directly above 
the case of the first formed sporocyst (p. 180, fig. 9). In this 
position, zoospores were formed and discharged in the usual 
manner. 
After the discharge of the second sporocyst, the process may 
be repeated so that a third sporocyst is formed on the top of the 
second; this, in turn, discharges its zoospores, leaving an empty 
terminal sporocyst. 
The hypha, which now completely fills the first and second 
sporocyst, may continue growth and fill the third so that the 
filament has exactly the appearance of a resting multiple sporocyst 
(p. 184, fig. 6; p. 170, fig. 11), and this seems to be the general 
method; or the second sporocyst may proceed to form zoospores 
and discharge them through the empty terminal one, and the same 
process may occur with the first, so that in this way a series of 
three empty sporocysts would be left, each clearly limited by a 
marked constriction (p. 180, fig. 11). 
In the figures, the walls of the sporocyst have been exaggerated 
for the sake of clearness, but in the actual cultures the hypha 
completely fills the sporocyst and cannot be distinguished from it 
when growth has ceased. 
The discharging process has been marked to show the limitation 
