182 
A. E Lechmere. 
of the sporocyst, but this is not seen in the actual cultures, as the 
sporocysts fit on to one another almost exactly, and it was only by 
watching the protruding end of the hypha that their formation in 
this manner was determined. 
In the case of the hypha filling the third sporocyst, a chain of 
three resting sporocysts, or “chain gemmae” is formed, having 
exactly the appearance of chain gemma formed in the ordinary 
way by simultaneous septation of several terminal segments (p. 184, 
figs. 6 and 7). On transferring the hyphae showing this condition 
to fresh water they form sporocysts which are discharged in 
basipetal succession, as in the case of S. torulosa, but in this case 
the sporocysts have no separate openings, each discharging through 
the one in front. 
At first this second method of new sporocyst formation seems 
in some ways to be exactly the reverse of the first method described, 
but, after the third sporocyst has been formed, the process is the 
same as that of the first, i.e., basipetal sporocyst formation. 
In old cultures the forms are very complicated and have various 
shapes. Several cases have been observed where, after a chain of 
three sporocysts has been formed, the hypha has continued growth 
as an ordinary filament (p. 184, fig. 4). 
Filaments in the older cultures showing this condition present 
somewhat the appearance of the constricted hyphae of the genus 
Apodya ( Leptornitus ), but no trace of constriction is found on the 
hyphae of young mycelia. 
No cases have been observed where more than three sporocysts 
were formed in this way. The older hyphae show sometimes several 
septae, but these are apparently formed simultaneously and represent 
a form of chain gemmae (p. 184, fig. 7). 
The third method of forming new sporocysts, and by far the 
more complicated, occurs in the case of the very elongated 
cylindrical form of first sporocyst. 
After the discharge of such a sporocyst, a new one grows up 
inside it, but terminates at or below the level of half its length 
(p. 180, fig. 4). In this position formation and discharge of zoospores 
take place in the normal manner. 
The empty case of the second sporocyst is now seen situated 
in the lower part of the first formed sporocyst. 
Soon the hypha recommences growth and fills up the empty 
case and protrudes in the manner previously described till it forms 
a third sporocyst immediately above the second, both being 
