Methods of Reproduction in the Saprolegniacece. 183 
contained within the original wall of the first. The third sporocyst 
is somewhat shorter in length than the second (p. 180, fig. 12). 
After the formation and discharge of this third sporocyst, the 
hypha continues its growth, filling the empty case and forming a 
fourth sporocyst in a similar manner to the third. 
This process can continue till a chain of four sporocysts is 
formed, all contained within the original elongated cylindrical 
sporocyst. After discharge of the small terminal sporocyst, or in 
case of its failing to discharge, the chain of sporocysts has exactly 
the appearance of a series of chain gemmae, and in this condition 
they can remain unchanged for some time (p. 184, fig. 1). 
On transferring to fresh water, the discharge takes place in 
basipetal succession (p. 184, figs. 3 and 5), each sporocyst discharging 
through the empty one in front of it. 
Old hyphae frequently present a very complicated appearance, 
as the portion of the hypha below the original terminal sporocyst 
shows septation into a number of segments more or less constricted 
(p. 184, figs. 2 and 3). 
The formation of new sporocysts in this species seems to be 
carried out with great persistence. Cases have been observed 
where, after the successive discharge of three sporocysts formed 
within the original terminal one, the hypha has again protruded 
into the empty case of the lowest of the chain, i.e., the second in 
the process of formation, and produced a small sporocyst with 
zoospores, and, at the same time, a segment has been cut off 
immediately below it, having the appearance of a sporocyst at an 
early stage (p. 184, fig. 5). In this case there is the possibility of 
nine discharges taking place, if the last formed sporocyst succeeds 
in producing zoospores, but the case figured was never seen to 
discharge, so that not more than seven actual discharges have 
occurred. 
After the discharge of a chain of sporocysts the old hyphae 
often present the appearance of repeated segmentation for some 
distance below the terminal sporocyst. Alternate segments of this 
part of the hypha are seen to be densely filled with granular 
protoplasm, while the other parts appear quite empty. These 
segments have exactly the appearance of “ resting sporocysts,” and 
in this way may be considered as intercallary gemmae. Their 
germination has not been observed (p. 184, fig. 3). 
The Presence of Gemmae. Gemmae of various shapes were 
observed at all stages of the cultures after a few days’ growth. 
