256 
S. Reginald Price. 
appearance of a piece of the stem with sclerotia, is shown in the 
photograph (Fig. 1), slightly reduced. 
Some of the sclerotia when teased out and examined under 
the microscope, showed thick-walled spherical spore-like bodies, 
very much larger than the conidia, and borne quite near the 
surface of the stroma : other sclerotia were bearing conidia alone. 
Although I have not succeeded in inducing these spore-like bodies 
to germinate, their characters strongly suggest that they function 
as spores. 
The wall of this peculiar spore-form is thick, as already stated, 
brownish yellow in colour, and slightly granulated on the exterior; 
while the contents are dense and granular with some oil globules. 
The spores are borne on septate hyphae, which project from the 
body of the sclerotium (Figs. 2 and 3). In general these hyphse 
resemble those which bear the conidiophores at their apices. 
Although the spherical spores are usually terminal, this is by no 
means invariably the case. Sometimes the hypha appears to grow 
again from the apical end of the spore, so that the latter assumes 
an intercalary position (Fig. 4). In other cases the free end of 
the hypha may form another spore, thus producing an intercalary 
and a terminal spore, separated by a short hyphal filament (Fig. 5). 
Others again show two spores in contact and the same arrange¬ 
ment as the last, but without the intervening hypha (Fig. 6). 
The measurements are as follows :— 
Diameter of spores - - 35-71 /x 
(According to relative maturity, etc.) 
Average diameter of fully ( 
developed spores ) 
Average thickness of hypae f 
bearing the spores j 
Hanging drop cultures were made in pure water, and in “ grape- 
extract,” using the spore-forms as free as possible from conidia, 
60 - 70 (i 
16 - 18 /a 
Fig. 1. Portion of stem of Conium , with black sclerotia. About f 
natural size. 
Fig. 2. Portion of sclerotium showing resting spores and hyphse. x 
100 diam. 
Figs. 3—8. x 350 diam. 
Fig. 3. Single terminal spore showing nature of wall and contents. 
Fig. 4. Spore in intercalary position. 
Fig. 5. Two spores separated by single hyphal cell. 
Fig. 6. Another common arrangement of two spores.. 
Fig. 7. Typical “organ of attachment” produced on germination of the 
conidia. 
Fig. 8. The same. Younger stage. 
