342 
E. K. Hanson. 
(l>). Weight of substance used, 04855 gms. 
Vol. of N. at 15°C and 776 mm. was 7-2 c.c. 
Whence per cent. N. = 4.65. 
The ash in (a) was not determined, owing to lack of 
substance. ( b ) contained 17%. 
These results probably indicate that the samples (a) and (i), 
which were made at different times, were mixtures of unlike 
composition. 
Both percentages are too low for true proteins, but the middle 
fractions give no higher, though more consistent, results. 
(c) . Weight used 0-172 gms. 
Vol. of N. at 17°C and 750 mm. = 5-3 c.c. 
Whence per cent. N. = 3-53. 
(d) . Weight used 0-2594 gms. 
Vol. of N. at 12°C and 763 mm. = 7-2 c.c. 
Whence per cent. N. = 3-31. 
Some of ( c ) was dried at 100°C to constant weight and 0-1630 
gms. lost 0-0248 gms., making per cent, moisture 15-8. Further, 
0-1438 gave 0-0390 ash = 27-1%. 
Determinations of carbon and hydrogen gave the following 
results:— 
On sample ( b ) (first fraction) 0-1775, gave 0-2440 gms. C0 2 
and 0-1215 gms. of water, or 37-48% C and 7-60% H. On a sample 
prepared in a similar way to ( c) and (d), 0-1862 grms. gave 0.2090 
C0 2 and 0-1290 grms. H 2 0 or 30-61% C, and 7-10% H. 
These figures are, however, obtained on material which is 
probably far from pure, and can only he regarded as provisional. 
The action of various reagents on phycoerythrin solution has 
been studied by Molisch (loc. cit.) and by Schiitt (loc. cit.): these 
observations have been repeated and extended. The following is a 
summary of results obtained :— 
(1) . Phycoerythrin is preciptated from aqueous solution by 
alcohol, or by saturating the solution with ammonium sulphate or 
with magnesium sulphate. It is precipitated by very small 
quantities of mercuric chloride. 
(2) . Dilute acids, added gradually, first cause disappearance 
of the orange fluorescence, leaving a lilac-pink tint, somewhat 
opalescent, and finally, after a couple of days, a pink precipitate 
settles down. 
(3) . Ammonia in a small amount removes the fluorescence, 
leaving a pink colour. Large excess turns this colour to a yellow- 
brown—similar to that caused by caustic soda, 
