t6 
F. T. Brooks and S. R. Price. 
similar to the manner in which other fungi develop their fructi¬ 
fications on various media, but this tendency was more marked in 
the case of certain cultures of the fungus originally derived from 
the stems of tomato plants. 
On pure agar the fungus grew much less readily ; the mycelium 
was sparse and only a few pycnidia developed. Fig. 1 represents 
Fig. 1. Fungus isolated from Fig. 2. Fungus isolated from 
fruit. Section of pycmdium on fruit. Fresh spores from pycnidium 
agar. xl25diam. on agar. x770 diam. 
a microtomed section of one of these pycnidia and Fig. 2 fresh 
spores from such a pycnidium. 
Cultures of the Fungus Isolated from the Stem. 
Cultures were obtained in the same manner as in the case of 
the fungus isolated from the fruit. The spores germinated readily 
both in water and in a dilute solution of sugar. 
On tomato-gelatine a dense white mycelium developed which 
began to sink into the medium in about 8 days and caused lique¬ 
faction of the gelatine. No fructifications were formed in cultures 
on this medium. 
On tomato-agar the mycelium grew less vigorously but numerous 
pycnidia developed. In appearance and structure these fructi¬ 
fications were identical with those which occurred in cultures of 
the fungus derived from the fruit. The spores, represented in 
Fig. 3, were similar and in old cultures were aggregated in the 
same manner at the orifices of the pycnidia. In these cultures 
also there was a tendency for the fructifications to develop in 
concentric circles and in one particular series of plates which were 
kept in a cool position in a North light during December this 
distribution was well marked, as is shown in Fig. 11, which is a 
photograph of one of these cultures. Although many other cultures 
were subsequently kept under approximately the same conditions, 
the “fairy ring” mode of distribution of the pycnidia was not again 
so well marked. 
On pure agar the mycelial growth was very thin and only a 
