A Disease of Tomatoes. 17 
small number of pycnidia were formed. Fig. 4 represents a micro- 
tomed section of one of these pycnidia. 
The above observations make it clear, therefore, that as regards 
behaviour on culture media the fungi derived from the fruit and 
stem respectively are identical. 
Fig. 3. Fungus isolated 
from stem. Fresh spores 
from pycnidium on tomato 
agar. x770 diam. 
Fig. 4. Fungus isolated from stem. Section 
of pycnidium on agar. x770 diam. 
Inoculation Experiments with Fungus Derived 
from the Fruit. 
Experiments showed that with green fruits infection would 
only result if the fungus, either in the form of mycelium from a 
pure culture on tomato-gelatine, or of spores, was inserted into 
wounded parts. In such inoculations a rot began in the immediate 
neighbourhood of the wound a few days after the fungus was 
Fig. 5. Fungus isolated from fruit. Section 
of pycnidium on an inoculated fruit, x 500 
diam. 
Fig. 6. Fungus isolated 
from fruit. Fresh spores 
from pycnidium on an 
inoculated stem. x 770 
diam. 
inserted and gradually spread throughout the tissues of the fruit. 
After 10-14 days numerous pycnidia developed on the surface. 
Fig. 5 shows a section of one of these pycnidia. The fruits remained 
