1 8 
F. T. Brooks and S. R. Price. 
sound in control experiments. With ripe fruits, however, experiment 
showed that infection sometimes resulted when the mycelium was 
placed on uninjured parts. 
Tnoculations of wounded parts of stems of tomato plants were 
also made with the same mycelium. For this purpose a longitudinal 
slit was made in the stem about an inch above soil level and some 
mycelium was inserted, after which the wound was covered with 
tinfoil. All inoculations were performed as far as possible under 
Fig. 7. Fungus isolated from stem. Fig. 8. Fungus isolated from stem. 
Fresh spores from pycnidium on Fresh spores from pycnidium on 
an inoculated stem. x770 diam. stem infected in nature. X 770 diam. 
sterile conditions. Other plants were kept as controls. The foliage 
of nearly every plant thus inoculated began to wilt about 7-8 days 
after the fungus was inserted. Fig. 12 shows one of the wilted 
plants and one of the control plants side by side. The affected 
plants subsequently died and in some of them pycnidia of the same 
character as those previously described, developed in the neighbour¬ 
hood of the wound. Fig. 6 shows spores obtained from one of 
these pycnidia. In connection with these inoculations it was noticed 
Fig. 9. Fungus isolated from stem. Section of young pycnidia on an 
inoculated fruit. X 125 diam. 
that pycnidia were not formed so abundantly as in inoculation 
experiments made with the fungus originally derived from tomato 
stems. To make the story complete the fungus was plated out 
from these pycnidia and in cultures it proved to be identical with 
the fungus which had been used for inoculation. Upon examination 
of the tissues of plants which were successfully infected the 
