270 -S. Reginald Price. 
I. POLYPORUS SQUAMOSUS IN ARTIFICIAL CULTURE. 
(a). The establishment of pure cultures on blocks of wood. 
Attempts were made to raise further cultures on Elm wood and to 
follow the development of the mycelium from the spores. 
The method employed was generally that used by Marshall 
Ward (14) for Stereum hirsutum, and by Brooks (4) for Stereum 
purpureum. 
By making use of the spore-cloud formation of the fructifications 
as described by previous investigators (3, 6), pure spore-deposits 
were obtained on sterilised glass slips, the spores being transferred 
to the blocks by means of a platinum needle. 
Up to December 6 there was no sign of any mycelial growth 
observed on the blocks inoculated with spores on October 23. It 
was hence concluded that the cultures had failed, so that some 
were thrown away. When the cultures were next examined, 
however, on January 17, 1912, a well marked mycelial development 
was obvious. As the mycelium was similar in all nine cases, and 
also similar to those begun in August, 1911, it was concluded that 
this was the mycelium of P. squamosns —which, however, took about 
two months to manifest itself. 
Mycelial transfers to other culture tubes grew slowly, a month 
being required for the growth of a thin layer. The mycelium 
spread gradually and grew in thickness, till after three or four 
months a dense white “felt” covering the whole outer surface of 
the block was produced (PI. VI, Fig. 2). At about this stage the 
felt became tinged with a pale brown colour exhibiting at the same 
time a dusty appearance ; this coincided with the production of 
“ oidia.” Soon after this stage the mycelium usually began to 
exude glistening drops of liquid from its surface which were at first 
colourless, but subsequently assumed a light brown tint, the mycelium 
also usually becoming darker brown at this stage. The liquid drops 
increased in size and ran together and often a few cubic centimetres 
of the liquid collected in the culture tube. Experiments showed 
that this liquid was water, coloured by some decomposition product. 
Similar exudations of water by the ripe fruit bodies of Polyporus 
and other fungi often take place (3, p. 850). 
Only on Elm wood were spore cultures made. Mycelial 
transfers were made to the following kinds of wood—Lime, Sycamore, 
Horse Chestnut and Pinus. Copious growth occurred on the wood 
of Lime, Sycamore and Horse Chestnut, but only a very sparse 
development of the mycelium took place upon that of Pinus (PI. VI, 
Fig. 3). 
