118 
PROFESSOR P. F. FRARKLAND AND MRS. G. C. FRANKLAND ON 
ture of other organisms than anything we had previously dealt with, and these 
were, therefore, made the basis for further attempts to isolate the nitrifying 
orcfanisms. 
One of these bottles, which had been inoculated from the gelatine-tube as above, and 
which had duly nitrified, was transmitted through four generations of culture in bottles 
containing sterile ammoniacal solution. Thus from the gelatine-tuhe inoculated on 
July 7, 1888 :— 
I. Bottle containing ammoniacal solution was inoculated from above July 12 , 1888 ; 
examined and found reactions with diphenylamine and sulphanilic acid, 
September 8, 1888. 
II. Bottle inoculated from I. bottle, September 3, 1888 ; examined and found reactions 
for nitrification, October 1, 1888. 
III. Bottle inoculated from IL bottle, November 11, 1888; examined and found 
reactions for nitrification, February 26, 1889. 
This III. bottl e was made the starting point for the following experiments :— 
Dilution Experiments, Series V., commenced March 20, 1889. 
One drop from the nitrifying solution in III. bottle referred to above, was diluted 
with 50 c.c. of sterile distilled water, giving the 
First Dilution . 1 drop = ^Jon of original drop. 
1 drop = of original drop. 
No. 1 bottle inoculated with 1 drop (= i.ooho'oo of original drop). 
No. 2 bottle ditto. 
No. 3 bottle ditto. 
Second Dilution 
(1 di’op of First 
Dilution mixed 
with 50 c.c. sterile 
water). 
No. 4 bottle inoculated with 2 drops (= WcTo^o'o of original drop). 
No. 5 bottle ditto. 
No. 6 bottle ditto. 
No. 7 bottle inoculated with 3 drops (= 1,000.000 of original drop). 
No. 8 bottle. ditto. 
No. 9 bottle ditto. 
I 
L 
x.oEroF o of original drop). 
No. 10 bottle inoculated with 10 drops ( = 
No. 11 bottle ditto. 
No. 12 bottle ditto. 
