THE NITRIFYING PROCESS AND ITS SPECIFIC FERMENT. 
119 
1 drop = g-o;^oV.oFo‘ of original drop. 
No. 13 bottle inoculated witli 1 drop (= of original drop). 
No. 14 bottle ditto. 
No. 15 bottle ditto. 
No. 16 bottle ditto. 
No. 17 bottle inoculated with 3 drops (= soTo fo.ooo of original drop). 
No. 18 bottle ditto. 
No. 19 bottle ditto. 
No. 20 bottle ditto. 
No. 21 bottle inoculated with 5 drops (= 50 . 000 , 000 ' of original drop). 
No. 22 bottle ditto. 
No. 23 bottle ditto. 
No. 24 bottle ditto. 
No. 25 bottle inoculated with 10 drops (= 50.000.000 of original drop). 
No. 26 bottle ditto. 
No. 27 bottle ditto. 
No. 28 bottle ditto. 
No. 29 bottle inoculated with 2 c.c. (= 50 , 000,000 of oi'iginal drop). 
No. 30 bottle ditto. 
No. 31 bottle ditto. 
No. 32 bottle ditto. 
A few of the above bottles were examined for nitrons and nitric acids on May 4, 
1889, or about six weeks after their inoculation, when the following results were 
obtained : — 
Bottle No. 10 (dilution Y7(fSoNoo) strong reactions with both diphenylaniine and sulphanilic 
acid. 
Bottle No. 1 (dilation i.ook 'ool)) distinct reactions. 
Third Dilution 
(1 c.c. of Second 
Dilution mixed 
with 50 c.c. sterile 
water). 
Further bottles wmre similarly examined on May 7tb, 1889 :— 
Bottle No. 13 (dilation 50 , 00 ^ 0 , 000 ) gave no reactions more than a blank bottle similarly preserved. 
Bottle No. 14 (dilution 50 , 00 ^ 0 , 000 ) ditto. 
Bottle No. 15 (dilution fo. 000 . 000 ) 
Bottle No. 2 (dilution 1 : 500 , 000 ) ditto. 
Bottle No. 11 (dilution y,o'5^5b7o'o) gave slightly stronger reactions than the blank bottle. 
Bottle No. 12 (dilution i,ook°,o6o ) gave very strong reactions with both reagents. 
Further examinations, both chemical and microscopical, were made on the following 
day. May 8th, 1889, with the following results :— 
