Table of Contents 
Introduction and Instructions.n 
Scientific Reports 
Pathogenic bacteria of insects as HVI systems 
by Robert Faust. 1 
(})do: Helper-independent temperate phage molecular 
cloning vehicles for Bacillus subtilis 
by Donald H. Dean, et. al .9 
Actions 
Issues pending. 15 
Cloning of DMA from higher eukaryotes; cloning into invertebrate and 
vertebrate cells in culture; lower eukaryotes as HV systems; groups of 
exempt exchangers; nonexchangers and physical containment; B. subti- 
lis as an HVI system; P3 + EK1 as alternative containment; cloning in 8, 
subtilis and S. coelicolor; single-strand bacteriophages as EK1 vectors. 
Issues resolved. 16 
■'Cosmids" as EK1 and EK2 vectors; male-specific baceriophages and 
EK1 containment; dA-dT tailing with certified EK2 vectors; x2282 in EK2 
studies; the certification of EK2 host-plasmid systems 
News and Comment 
The new RAC. 19 
COGENE. 21 
COGENE reports on (i) risk assessment, (ii) recombinant DNA Guide¬ 
lines and (ill) the benefits and application of recombinant DNA technol¬ 
ogy 
Technical Information 
Host-vector systems. 25 
Characterized clones. 28 
Announcements 
International Conference on Recombinant DNA; future RAC meetings; 
Laboratory safety course; ... 31 
Bibliography. 33 
(i) 
