REDUCTION OF CONTAINMENT LEVELS OF OHARACTERIZED RECOMBINANT DNA CLONES 
BY NIH REOOMBINANT DNA ADVISORY OOMMITTEE 
CLONE 
NUMBER 
ORIGIN 
OF 
CLONE 
CONTAINMENT 
APPROVAL FOR 
CHARACTERIZED CLONE 
DATE 
APPROVED 
BASIS OF 
CHARACTERIZATION 
LITERATURE 
REFERENCES 
K149 
Mouse Ig 
cDNA kappa 
light chain 
P2 + EK1 
8-10-78 
In situ 
hybridization, 
R-loop analysis, 
sequencing 
1 
K41 
Mouse Ig 
cDNA kappa 
light Cham 
P2 -r EK1 
8-10-78 
In situ 
hybridization, 
R-loop analysis, 
sequencing 
2 
M/3G2 
Mouse 
beta globin maj 
genomic 
P3 + EK1/P2 + EK2 
8-10-78 
In situ 
hybridization, 
restriction site 
analysis, 
R-loop analysis 
3 
M/3G3 
Mouse 
beta globin min 
genomic 
P3 + EK1/P2 + EK2 
8-10-78 
4 
MK2 
Mouse Ig 
kappa light chain 
genomic 
P3 + EK1/P2 + EK2 
8-10-78 
5 
MK3 
Mouse Ig 
kappa light chain 
genomic 
P3 + EK1/P2 + EK2 
8-10-78 
6 
Mol 
Mouse 
genomic 
alpha globin 
P3 -r EK1 /P2 + EK2 
8-10-78 
" 
7 
POvE12 
Chicken 
ovalbumin 
P2 + EK1 
8-9-78 
Restriction 
analysis, 
hybridization 
8 
PAFP5 
Rat 
alpha fetoprotein 
P2 -r EK1 
8-9-78 
Hybridization, 
gel analysis, 
restriction 
analysis 
9, 10 
pCg13 
pCg45 
Chicken 
procollagen 
P2 + EK1 
9-19-78 
Restriction analysis, 
hybridization, 
sequencing 
11 
pA2b(11)7 
p/<(11)24 
Mouse Ig 
heavy chain 
Mouse Ig 
light chain 
P2 + EK1 
8-9-78 
Restriction analysis, 
hybridization 
12 
pa(558)13 
PLi(3741)9 
Mouse Ig 
heavy chain 
Mouse Ig 
light chain 
P2 + EK1 
8-9-78 
Restriction 
analysis 
13 
pHol 
pH/3G1 
pHVGI 
Human 
cDNA 
globin 
P2 + EK1 
10-12-78 
Hybridization, 
sequence [partial] 
analysis 
14 
3Hb117 
Mouse globin 
P2 + EK1 
10-27-78 
Hybridization, 
sequencing 
15 
PXIvc 2,8, 
10-13, 
18-24, 
27-30, 
37,72, 
73 
Xenopus 
laevis 
vitellogenin 
cDNA 
P2 + EK1 
12-12-78 
R-loop analysis, 
hybridization 
purity of starting 
material 
16, 17 
pHb1008 
Chicken 
alpha globin 
cDNA 
P2 + EK1 
12-18-78 
Restriction mapping, 
sequence analysis. 
18 
pH21 -1 
Mouse Ig 
heavy chain 
cDNA 
P2 + EK1 
12-18-78 
Restriction analysis, 
nucleotide sequence 
analysis 
19 
pS/32B 
PS/3C69 
pSa56 
Sheep globin 
cDNA 
P2 + EK1 
12-19-78 
Hybridization, 
restriction analysis, 
purity of starting 
material 
20, 21 
