G. H. F. Nuttall and H. B. Fantham 
125 
of corpuscles containing different numbers of parasites. The results are 
given in the following Table: 
TABLE III. 
Showing the percentages of infected r.b.c. and the relative numbers of infected r.b.c. 
containing one to eight parasites. The percentages are based on a count of 1000 r.b.c. 
made from each preparation excepting in the case of brain and marrow smears where, 
owing to few r.b.c. being present, only 500 corpuscles were counted. The blood films 
and organ smears were made immediately after the death of Cow I. 
Blood from 
jugular 
Heart 
Lung 
Liver 
Spleen 
Kidney 
Supra¬ 
renal 
Brain 
Bone 
marrow 
°/ 0 infd. r.b.c. 
73-3 
69-7 
71-2 
74-8 
72 8 
759 
75-6 
73-2 
84 - 4 * 
r.b.c. containing 
1 parasite 28 - 2 
25T 
31-4 
29 
27 
29-5 
28-8 
31-8 
33 
2 parasites 
29 T 
25-8 
24-3 
27 
27 
26-8 
30-5 
23-8 
29-2 
3 „ 
9-4 
12-6 
9T 
11 
9-3 
11 
9-2 
11-6 
12 
4 „ 
5-4 
4-9 
4-8 
5-7 
6-4 
6-6 
5 
4 
6-6 
5 
7 
1-9 
1-3 
1 
1-6 
•9 
1-1 
1-4 
1-6 
6 „ 
5 
•9 
•3 
•8 
1-2 
•7 
•6 
0 
1-2 
7 „ 
0 
•4 
0 
•2 
•2 
•2 
•2 
0 
•2 
8 „ 
0 
T 
0 
T 
•1 
•2 
•2 
•6 
•6 
* This count is probably too high owing to the pale staining of many uninfected r.b.c. 
which may consequently have been overlooked. 
Conclusions. 
(1) The best results in the staining of these minute parasites are 
obtained by the ordinary method of drying the blood films, fixing them 
in absolute alcohol and staining them according to Giemsa. Methods of 
wet fixation give poor results, because the corpuscles do not become 
flattened as in drying, the consequence being that the haemoglobin clouds 
the image of the parasites. 
(2) The percentage of infected corpuscles in the peripheral circula¬ 
tion rises steadily as the disease progresses and at the same time there 
is a progressive increase in the proportion of infected corpuscles which 
contain two, three and four or more parasites. 
(3) The percentage of infected corpuscles observed in smears from 
various iniernal organs immediately after death, and the percentage of 
corpuscles containing more than one parasite, coincide with those 
obtained in the case of the peripheral blood. In other words, there 
does not appear to be a heavier infection of the corpuscles in any of the 
internal organs than there is in the general circulation. 
