Volume III 
JULY, 1910 
No. 2 
THE1LERIA EAR FA, THE PARASITE OF EAST 
COAST FEVER IN CATTLE. 
OBSERVATIONS ON STAINED PREPARATIONS. 
By GEORGE H. F. NUTTALL, F.R.S., 
AND H. B. FANTHAM, D.Sc. (London). 
(From the Quick Laboratory, Cambridge.) 
(Plate XII and 2 Charts.) 
In an earlier paper (Nuttall, Fantham and Porter, 1909, Parasitology ( 
vol II. pp. 325—340), we recorded our observations on living Theileria 
parva as seen in the peripheral blood of two cows which succumbed to East 
Coast Fever 1 . We now propose to describe our studies upon the parasite 
in stained preparations made from the animals’ blood during the course 
of the disease and from their organs shortly after death. We shall 
confine our attention to the types of parasites encountered within red 
blood corpuscles or to corresponding types which may be occasionally 
encountered free in the plasma. The subject of “Koch’s blue bodies” 
will receive attention at a later date. 
Methods used in making preparations. 
% 
Blood films were prepared daily from the cows, beginning a few days 
after the pathogenic ticks ( Rhipicephalus evertsi) had been placed upon 
the animals. 
The blood was obtained in the usual way by puncturing an ear- 
vein; it was usually spread thinly on slides, rapidly air-dried, fixed 
in absolute alcohol and stained by Giemsa. We preferred this method 
of fixation to wet fixation methods because the latter yielded inferior 
1 For further details regarding the two cows above-mentioned, the reader is referred to 
Parasitology, vol. n. pp. 208—210 and p. 328, 
Parasitology in 
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