204 Degenerative Appearances in Protozoa 
of Piroplasma in the papers referred to above. The remaining figures 
(Figs. 5—-12) show the appearances of the parasites in the same animal 
six hours after the injection of a curative dose of trypanblue. In Fig. 5 
we have a common type, the parasites being irregular or rounded in 
form and shrunken. Pairs of pyriforms are no longer encountered or 
they are very scarce. Where two parasites occur in a corpuscle they 
appear rounded or irregular (Figs. 7, 8). In Fig. 8 the chromatin is drawn 
out like a flagellum to one side, reminding one of certain parasites 
which various authors have wrongly regarded as flagellate forms of 
Piroplasma. In most parasites the protoplasm stains faintly blue, and 
frequently, as is shown in the figures, the chromatin assumes fantastic 
shapes and is extruded. Figs. 6 and 7, 9 and 10, 11 and 12 represent 
pairs of corpuscles containing degenerating parasites and lying side by 
side in the blood-film from which the drawing was made. 
In untreated dogs, it is rare to encounter parasites extruding their 
chromatin. We have noted this elsewhere (Nuttall and Graham-Smith, 
1906, Journ. of Hygiene, VI. p. 597, Diagram 7; p. 598, Diagram 9, 
Figs. 21, 23). ” 
II. The degeneration observed in Trypanosoma brucei consequent upon 
Arsenophenylglycin treatment. 
The effect of arsenophenylglycin upon Tr. brucei in treated mice is 
very marked, and is recorded in the following tables. The dose of the 
drug administered subcutaneously was 1 c.c. of a 1:70 aqueous solution 
per 20 gramme mouse. 
Mouse I. 
% of tryps. 
showing 
% of tryps. containing 
Time 
to o 
U Sh 
O 
<*- o 
o 53 
No granules 1 
1 
Granules 
/ 
1-2 granules \ 
3-5 granules 
i 
Many gran¬ 
ules ) 
% pale 
% rounded 
or breaking 
up 
% pointed 
% blunt 
Before treatment: 
1 hr. 
12 
95 
5 
4 
0 
1 
0 
0 
92 
8 
After treatment: 
1 hr. 
10 
5 
95 
19 
44 
31 
1 
0 
0 
100 
2 hrs. 
8 
0 
100 
4 
22 
69 
1 
4 
0 
96 
3 „ 
0-6 
0 
100 
2 
16 
62 
11 
9 
0 
91 
