Gr. H. F. Nuttall 
207 
granular, the other rounded and breaking up. After t hours only 
one parasite was found per 30,000 r.b.c. enumerated; this parasite was 
pale, rounded and breaking up. 
Treated Mouse II. 
o/o of tryps. °/o of tryps. 
showing containing 
lh d 
CO 
<13 
CO 
03 
CO 
03 
G 
& 
bo 
£■5 
■s 
03 
-4J o 
t*_ o 
°s 
G 
cd 
So 
03 
G 
c3 
CO 
3d 
P 
G 
ci 
s> 
3d 
03 
TS 
ft 
s s 
3 t- 
OJ2 
.5 c 
0-3 
S'® 
G.S 
O 
U 03 
Parallel observations 
on the living 
Time 
O 03 
sl 
6 
eo 
I s 
o 
®~ 
-=0 3 
-S’ 0 
0 
parasites 
Before treatment, 
18. ii.’10 : 
10.40a.m. 10 
86 
14 f * 
8 
4 
2 
0 
0 
100 
0 
— 
After treatment: 
i hr. 
10 
28 
72 ffe 
10 
30 
32 
2 
0 
18 
82 
Tryps. more active 
than normal. 
1 „ 
7 
2 
98 fc 
4 
40 
52 
0 
2 
16 
82 
Still more active. 
1| hrs. 
6-5 
0 
100 fee 
0 
10 
90 
14 
6 
2 
92 
Less active, appear 
granular. 
2 „ 
5 
6 
94 ffe 
8 
26 
60 
48 
6 
0 
94 
Ditto. 
4 
2 
98 fffc 
2 
18 
78 
94 
18 
0 
82 
Ditto, but more 
,, 
granular. 
3 „ 
4 
42 
58 fffc 
28 
16 
14 
100 
6 
0 
94 
Ditto. 
H „ 
2-5 
44 
56 fffc 
22 
24 
10 
100 
14 
0 
86 
Ditto. 
4 „ 
1-0 
0 
100 
0 
0 
100 
100 
12 
0 
88 
Still less active, 
granular. 
4 J „ 
0-16 
0 
100 
0 
4 
96 
100 
• 
0 
• 
Ditto. 
5 „ 
0-14 
0 
100 
0 
16 
84 
100 
16 
0 
• 
Much degenerated, 
very granular, 
flagellum moving 
slowly. 
!> 
0-13 
0 
100 
• 
• 
• 
• 
• 
• 
• 
Ditto (only 2 tryps. 
seen). 
6 „ 
0-06 
. 
• 
• 
• 
• 
• 
• 
• 
• 
Ditto (ditto). 
61 „ 
0-06 
• 
• 
• 
• 
• 
• 
• 
• 
• 
No tryps. found. 
7 „ 
0-03 
• 
• 
• 
• 
• 
• 
• 
• 
• 
Ditto. 
* f= 
granules 
all 
fine, fc: 
= granules 
growing 
coarser 
, ffe 
=granules mostly fine, 
fee=granules mostly coarse, fffo = granules mostly very fine. 
Owing possibly to the intense staining process to which the blood- 
films were exposed a somewhat lower percentage (86 °/ 0 ) of the trypano¬ 
somes was found to be free from granules in Mouse II. before treatment 
was commenced. Following upon treatment the percentage of granular 
parasites rose rapidly, but after 3—3-|- hours many pale trypanosomes 
were noted which contained no granules. After 1-J hours no trypano¬ 
somes with pointed posterior extremities were enumerated. All of the 
parasites appeared pale after 3 hours, and after 2^ hours a considerable 
