208 Degenerative Appearances in Protozoa 
number appeared rounded or breaking up. As in the previous experiment 
the number of trypanosomes had begun to decrease already 1 hour 
after the drug was injected. It will be noted that the granules grew 
coarser up to 1^- hours after treatment and subsequently appeared to 
grow finer. In a number of cases the coarser granules took on almost 
a blackish tint and were an’anged in rows in the degenerating trypano¬ 
somes. 
The trypanosomes, viewed under the microscope in fresh blood, 
showed increasingly active movement up to 1 hour after the drug was 
injected, but after 1^ hours they appeared granular and their move¬ 
ments grew slower. After 5 hours only the flagellum was seen to move 
slowly. Whereas there were 10 trypanosomes present per 1000 r.b.c. 
before treatment, there was but 1 trypanosome per 30,000 r.b.c. present 
7 hours after treatment. 
In the case of the untreated mouse it is interesting to note that 
the percentage of granular typanosomes increased rapidly on the day 
on which the animal died. The proportion of trypanosomes containing 
many granules also increased as the time of death approached. Four 
hours before the mouse died 12 °/o of the trypanosomes no longer showed 
pointed extremities. It appears therefore as if similar degenerative 
appearances occur in trypanosomes both in treated and untreated 
animals, in the latter case only on the approach of the host’s death. 
Untreated Mouse (Control to Mouse II.). 
% Of tryps. % of tryps. 
showing containing 
W O 
&■? 
CO 
© 
'p 
m 
a> 
to 
<v 
P 
d 
bo 
rs P 
33 
<D 
'P 
Time 
18. II. ’10 
■+* O 
«♦- o 
oo 
ll 
P 
d 
H 
bO 
O 
fc 
Granule: 
P 
d 
u 
bo 
P 
d 
Si 
bo 
to 
CO 
Many gr 
ules 
<D 
73 
o 
o' 
p 83 
P Si 
O fP 
s< . ^ 
1® O P 
o 
% Point 
behind 
si 
o2 
5- 1 <d 
o 
10.40 a.m. 
2 
74 
26 
10 
6 
10 
0 
0 
100 
0 
2.30 p.m. 
11 
90 
10 
2 
4 
4 
0 
0 
94 
6 
4.30 p.m. 
10 
90 
10 
10 
0 
0 
0 
0 
98 
2 
6 p.m. 
10 
98 
2 
2 
0 
0 
0 
0 
98 
2 
19. ii. ’10 
9.45 a.m. 
48 
22 
78 
24 
28 
26 
0 
0 
100 
0 
1 p.m. 
24 
2 
98 
0 
28 
70 
0 
0 
88 
12 
5 p.m. Mouse died. 
The observations above recorded indicate that the following changes 
take place in the trypanosomes consequent upon the drug treatment:— 
They retract their “beaks,” and deep-staining reddish or purplish granules 
