N. H. SWELLENGrREBEL 
231 
appearance of a nuclear membrane (Fig. 2a, 2c); or two granules of 
the same size are present, each of them may be divided (Fig. 2 cl, 2e). 
No real nuclear membrane is to be seen. The peripheral chromatin 
cannot be considered as such, often it is only found at the two poles of 
the nucleus. The preparations fixed by this method were also stained 
with iron-hematoxylin. No structure of the achromatin of the nucleus 
was to be seen, the chromatic granules were embedded in a structureless, 
pale-grey matrix. As in Giemsa stained preparations one or more 
central granules and a more or less complete peripheral mantle of 
chromatin was to be seen (Fig. 2/’). 
Fig. 3. Fixation in Schandinn’s corrosive alcohol. Heidenhain’s stain. 
Fixation with Schaudinn’s fluid (sublimate and absolute alcohol) 
followed by Heidenhain’s stain gave the same results as alcohol fixation 
of dried films stained in the same way. The achromatin is without any 
structure. A central agglomeration of chromatin is present; at the 
15—2 
