N. H. S WELLBNGREBEL 
observed. This method of wet fixation is highly recommendable, because 
it possesses the advantages of Flemming’s and Hermann’s solutions 
without their disadvantages. Fig. 6a, 6cl show that the nuclear structure 
after Rath’s fixation is the same as that already described. Fig. 6a 
presents a very distinct division of the two chromatic granules. Giemsa’s 
Fig. 6. Bath’s fixation. Heidenhain’s stain. 
stain after Rath’s fixation does not give such bad results as are obtained 
by other methods of wet fixation, but they are inferior to those obtained 
with dried films. Rabl’s and Bouin’s liquids are not so useful as Rath’s 
but give rather good results. Delafield’s hematoxylin and Mayer’s 
hemalum gives mediocre staining after Bouin fixation. The nuclear 
structure brought out by these methods is similar to that already 
described. 
Merkel’s solution gives very inferior preparations. Not only a 
considerable shrinkage is to be observed, but no sufficient staining with 
iron-hematoxylin can be obtained. Only Ehrlich-Biondi’s mixture gives 
rather distinct nuclear figures, but the shrinkage is so considerable that 
the preparations cannot be trusted. 
Wet alcohol fixation with Giemsa’s or Heidenhain’s stains gives 
similar results to those obtained by other methods (Fig. 7). 
I could not obtain good preparations either with iron-hematoxylin, 
or with Giemsa’s stain after fixation in osmic acid (vapour or 2 °/ 0 solution) 
followed by hardening in absolute alcohol. My preparations showed 
only the general outlines, rarely the internal structure of the nucleus 
was to be seen. 
When comparing the results obtained by the different methods of 
fixing and staining I am unable to confirm Minchin’s statement that 
Giemsa’s stain is useless for the study of minute nuclear structure. On 
