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which may often be found to consist of two granules (Fig. 46). A closer 
examination shows that the two granules are embedded in an achromatic 
globule, coloured pale grey, but generally left uncoloured after differ¬ 
entiation in iron-alum, so that the blepharoplast seems to consist only 
of the chromatic rodlet or the diplosome. Perhaps it will be useful to 
point out again that this diplosome cannot be considered as a sign of 
division, the Trypanosomes being no more in a division period. 
Similar but more distinct results are to be obtained after Rath’s 
fixation and iron-hematoxylin. In some cases the blepharoplast has the 
shape of a rodlet as in Fig. 6e ; Fig. 6c shows, however, that only part 
of the blepharoplast had been stained in the foregoing figure. In Fig. 6c 
the blepharoplast is composed of an intensely staining rodlet partly 
surrounded by the achromatic substance. Fig. 6a, 6b present the same 
structure but the rodlet appears here as a diplosome. In Fig. 6d only 
a grey coloured globule is to be seen, not showing any internal structure, 
the colour has in this case been completely removed. 
Fig. 7. Alcohol fixation of never-dried preparations. Giemsa’s stain. 
Flemming’s and Hermann’s liquids are useless for the study of the 
blepharoplast, Giemsa’s stain after alcohol-fixation of dried films, wet 
alcohol fixation or fixation in osrnic acid is very useful to show the 
minute details of the structure of the blepharoplast (Fig. 2a — 2d, 
Fig. 4c, Fig. 7) because the chromatic and achromatic components are 
stained in different varieties of red. Care must be taken that the 
staining does not last too long, if this precaution is not observed 
chromatic and achromatic substances are stained with equal intensity 
and the blepharoplast looks in such cases like a large structureless 
globule, as has been figured by Minchin. Giemsa-stained prepai’ations 
produce the same structures of the blepharoplast as those stained by 
Heidenhain’s method as may be seen when comparing the text figures. 
Consequently it is evident that dried films fixed with absolute alcohol 
and stained by Giemsa present the same minute structure of the 
blepharoplast as do the different methods of wet fixation and staining. 
