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Trypanosoma leivisi 
The structure of the blepharoplast described here was already 
known from Prowazek’s and Wenyon’s (1908) work. Minchin asserts 
that the blepharoplast is structureless. 
3. The flagellum and its basal granule. Minchin states that the 
base of the flagellum does not reach the blepharoplast, when films are 
dried before fixation and that consequently a more or less extensive 
cleft may be observed between the two. In my preparations (dry 
fixation, Giemsa’s stain) the following peculiarities could be observed. 
Often the flagellum seemed to be joined directly to the blepharoplast 
(Fig. 2b — 2d)] in other cases the basal part of the flagellum was stained 
with less intensity than the other parts of the flagellum, so a chromatic 
and an achromatic part of the flagellum could be distinguished, the first 
being united to the blepharoplast by means of the latter (Fig. 2a, 2e). 
Sometimes, but by no means as a rule, the base of the chromatic 
flagellum seemed to be broadened there where the achromatic filament 
commences. I think that this bi'oadened end must be considered as the 
basal granule (Minchin’s blepharoplast). 
Iron-hematoxylin after fixation with corrosive alcohol gave similar 
figures but the achromatic filament uniting the basal granule and the 
blepharoplast (Fig. 4 a, 4 b) was but rarely observed. Generally the 
basal granule was superposed on the blepharoplast (Fig. 4 a, 4 b), some¬ 
times a free space was left between the two (Fig. 3a). The basal 
granule was clearly to be seen with Heidenhain’s stain even better than 
with Giemsa’s. The increase in size of the basal granule after Giemsa’s 
stain, as pointed out by Minchin, could never be observed. 
4. The Cytoplasma. After fixation by Flemming’s fluid a distinct 
alveolar structure of the cytoplasma may generally be observed. With 
other methods (dry fixation, corrosive-alcohol, Rath) no such structure 
can be seen, so I presume that it is an artefact. 
After wet alcohol-fixation the cytoplasma assumed a deep blue colour 
and did not completely fill the space enclosed by the periplast, leaving 
a large part of it uncoloured. The coloured and uncoloured parts were 
sharply separated (Fig. 7a). I think this structure may be identified 
with Minchin’s “ Periplast line.” The relation of the coloured and 
uncoloured part is not always such as I have here described. Sometimes 
the coloured part is confined to a narrow median space (Fig. 7b) and may 
be composed of two parallel longitudinal filaments (Fig. 7c). Here the 
“ contraction ” of the protoplasma is very intense, much more so than 
after Flemming’s fixation although considerable alteration of the cell 
form occurs in the latter case. It is therefore by no means clear why 
after such intense plasmatic contraction the outlines of the cell are so 
