344 
A NOTE ON THE SPIROCHAETE PRESENT IN ULCERATIVE 
GRANULOMA OF THE PUDENDA OF AUSTRALIAN 
NATIVES. 
By W. CECIL BOSANQUET, M.A., M.D., E.R.C.P. 
(From Prof. E. A. Minchins Laboratory of Protozoology, Lister 
Institute of Preventive Medicine ) 
(Five Text-figures.) 
Early in January of the present year Professor Minchin kindly gave 
me for examination a portion of tissue and a mounted section taken 
from a case of the above disease and sent him by Dr J. Burton Cleland, 
then of Perth, W. Australia. The tissue was already embedded in 
paraffin, and both it and the section were stained by Levaditi’s method 
and showed the presence of numerous spirochaetes. 
These organisms were situated deeply in the tissue, 3 or 4 mm. from 
the surface, in the fibrous layer forming the base of the ulcer, and were 
most numerous in the immediate neighbourhood of the blood-vessels of 
this part. A few were visible within the vessels themselves. The 
longest forms seen measured on an average 12 /a in length: they were 
so slender that their breadth was rather a matter of guess-work, but it 
may perhaps be put at \y. (Fig. 1). Shorter forms were common, 
ranging down to 3 or 4/r, in length : these or some of them may have 
been due to division of longer forms in cutting sections of the tissue. 
There were a few individual organisms which were somewhat thicker 
than the average (l^f). 
The spirochaetes were in some instances homogeneous throughout 
in appearance, but a considerable number presented at certain points in 
their length definite granules staining more deeply than the rest of the 
protoplasm (Fig. 2). In some cases these granules were distinctly 
greater in diameter than the rest of the organism. Terminal granules 
