W. 0. Bosanquet 
345 
of this latter kind were not uncommon : a few spirochaetes were seen 
possessing two well-marked granules near to one end (Fig. 3). In some 
specimens the whole protoplasm was granular throughout, so that the 
organism resembled a string of heads closely threaded: these were 
presumably degenerative forms. A few instances of abnormal forms 
were also seen— e.g., a thick specimen with a well-marked loop or 
vacuole near to one extremity ; a second in which a large terminal 
granule present was transparent and unstained, giving somewhat the 
appearance of a loop; and a few individuals coiled up into rings (Fig. 4). 
I found one or two instances of what appeared to be forked forms, 
suggesting longitudinal division, and Cleland also saw a few of these; 
but I do not feel at all certain that these specimens were really bifid, as 
it is difficult to eliminate possibilities of mere apposition of organisms 
when dealing with creatures of such tenuity. 
1 2 3 4 5 
The curls or waves in the spirochaetes were quite irregular, unlike 
the symmetrical curls of Treponema pallidum. Some organisms lay 
almost in straight lines: others were twisted on themselves, and others 
again were merely sinuous (Fig. 1). On the whole they resembled very 
closely the figures given by Castellani of his Spirochaeta, pertenuis of 
yaws, though the illustrations of this parasite given by Levaditi and 
Nattan-Larrier make it closely resemble T. pallidum. In a piece of 
yaws-granuloma very kindly sent me by Dr Castellani, I was unsuccessful 
in finding the parasites by the silver-method, as I had hoped to do for 
the sake of comparison. The spirochaetes in the present case of 
ulcerative granuloma did not resemble, except in slenderness, the 
illustrations given by Maclennan of this organism: possibly those from 
which his figures are taken were derived from the surface of the ulcers 
and may have assumed a more curved appearance, when thus lying free, 
than they present when compressed into the interstices of the tissues. 
No illustrations are given by Wise who first discovered spirochaetes in 
this disease. He originally found them in smears from the diseased 
tissues—some of them taken from the deeper parts. He does not, 
however, speak of having stained the organisms in sections of the tissue. 
