406 
Spirochaetes of Tapes aureus 
EXPLANATION OF PLATE VI. 
All figures were outlined with an Abb£-Zeiss camera lucida, using a Zeiss 2 mm. 
apoelxromatic or ^ inch achromatic objective and compensating ocular 8. 
The magnification is, in all cases, except Fig. 54, approximately 1300 diameters. 
Figs. 1—37 are arranged to show gradual increase in breadth of the various specimens of 
Spirochaetes found in Tapes aureus. 
Figs. 1—5. Very thin forms of Spirochaetes from Tapes aureus. The edge of the 
membrane is well shown, but the organisms are too thin for the internal chromatin 
apparatus to be seen clearly, except in a few areas. These thin forms are of variable 
length, and result from longitudinal division. The Spiroehaete drawn in Fig. 5 is 
probably about to divide transversely to produce a form similar to that shown in 
Fig. 1. (Iron-haematoxylin.) 
Figs. 6, 7. Slightly thicker forms, with rounded ends and variable length, in which the 
transverse bars of chromatin could be well seen in parts. (Twort’s stain.) 
Figs. 8, 9. Short, somewhat thicker Spirochaetes, with relaxed membrane, showing 
myonemes. The ends of the organisms are tapering. (Delafield’s haematoxylin.) 
Fig. 10. Thin Spiroehaete with pointed ends, showing clearly the transverse bars of 
chromatin. The membrane is probably very closely contracted round the body, and 
the edge is only seen at one point. (Iron-haematoxylin.) 
Fig. 11. Small (young) Spiroehaete with well-marked relaxed membrane showing 
myonemes. The membrane is slightly folded over at its upper end. The parasite 
occurred in a preparation in which there were many forms like those drawn in 
Figs. 29—32. (Delafield.) 
Fig. 12. Spiroehaete showing marked spiral coiling and slightly dissimilar ends. The 
edges of the membrane and body are not clearly distinguishable from each other. 
(Delafield.) 
Fig. 13. Bather long, relatively straight form, with one end pointed, the other end 
rounded. Membrane contracted against the body. (Delafield.) 
Fig. 14. Spiroehaete with many coils and rounded ends. Internal chromatin apparatus 
not well shown. (Delafield.) 
Fig. 15. Long Spiroehaete, with coils somewhat flattened. The ends are tapering and 
membrane closely contracted. (Iron-haematoxylin.) 
Fig. 16. Spiroehaete with tapering ends. Internal structure not very clearly seen. 
(Delafield.) 
Fig. 17. Shorter form with pointed ends. Produced by transverse division of such 
a form as shown in Fig. 15. (Iron-haematoxylin.) Note the variation in degree of 
the tapering of the ends in Figs. 13—17. 
Fig. 18. Long Spiroehaete with edge of membrane clearly stained lying close to or 
along the body. Ends somewhat rounded. (Iron-haematoxylin.) 
Fig. 19. Long Spiroehaete with pointed ends. Membrane not well-marked. The spiral 
character of the internal nuclear apparatus is seen near the upper end. (Iron- 
haematoxylin.) 
Fig. 20. Long Spiroehaete with pointed ends. The edge of the contracted membrane is 
seen at intervals. Some of the chromatin bars show signs of division at the upper 
end. (Iron-haematoxylin.) Many similar forms occurred in a preparation treated 
with gold-chloride. 
Fig. 21. Spiroehaete with pointed ends. (Iron-haematoxylin.) 
