412 Treatment of Canine Piroplasmosis 
to 80 minutes to the action of a 5 °/o solution of the dye proved to be 
non-iufective when injected into two dogs. 
Fowler s solution. Levi della Yida states that he treated one dog 
with - 01 to 0T5 gramme of Fowlei’’s solution per day. A few parasites 
appeared in its blood and the disease ran a mild course. This result 
may very well be attributed to the mild strain of piroplasmosis with 
which the author was working. In the absence of careful observations 
upon the parasites, and in the absence of control experiments, no con¬ 
clusions can be reached regarding the value of this mode of treatment. 
Sodium cacodylate. Levi della Vida states that he treated 7 dogs 
with this drug and that only one died of piroplasmosis. In the 6 dogs 
which recovered the drug was given in doses of 15 to 20 centigrammes 
per day during 4 to 6 days. In 5 dogs treatment began 48 hours after 
inoculation and in 1 dog 4 days after inoculation. Of the 6 dogs 3 
never showed parasites, whereas the other 3 suffered from a mild form 
of the disease. The three dogs in which parasites did not appear 
subsequently proved to be susceptible to infection through the agency 
of ticks (no particulars given). Blood mixed with a 1'8% solution of the 
drug in ‘9°/o NaCl solution and allowed to stand 2 hours at room 
temperature remained infective. In the absence of further details 
regarding these experiments, the most that can be said is that it 
appears expedient to repeat them. I propose to do this, and shall 
report at a later date upon tine results. 
Experiments with various dyes. Levi della Vida (1907, p. 859) found 
that Methylene blue exerted no effect upon the course of the disease in 
two dogs which received 4 centigrammes of the dye injected subcu¬ 
taneously as soon as the parasites appeared in the animals’ blood. 
(These results accord with those of Nuttall and Graham-Smith, 1908, 
p. 222.) 
Brilliant green injected subcutaneously at the same time as the 
inoculation was made did not lengthen the period of incubation nor 
retard the death of a dog thus treated, the dose administered amounting 
to ^ c.c. of a 0 - 5 % solution of the dye. A second dog received two 
subcutaneous injections, each of 1 c.c. of a 05 °/ 0 solution of brilliant 
green, after the parasites had appeared in its blood, but the dye exerted 
no apparent effect; the dogs died of piroplasmosis on the 9th day. 
(These results accord with those obtained in my experiment recorded 
below and carried out before I was aware of the experiments of Levi 
della Vida.) 
