U. IL. F. Nuttall 
413 
Conclusions regarding the Italian Experiments. 
None of the dyes tested by Levi della Vida could, therefore, in the 
light of his experiments, be regarded as of value in the treatment of 
canine piroplasmosis. The results obtained by this author and Memmo, 
Martoglio and Adani with other modes of treatment are either contra¬ 
dictory or doubtful. The experiments with sodium cacodylate require 
to be repeated. 
2. Further Experiments with Dyes. 
The following protocols relate to experiments which I have carried 
out with Brilliant Green, Benzopurpurine and Congo Red : 
Experiment with Brilliant Green. 
Dog 1 : Fox-terrier puppy. Weight 9 lbs. 
Received one subcutaneous injection of Brilliant Green in 1 °/ 0 watery solution 
24 hours after the parasites first appeared in the dog’s blood x . 
Date Day Temp.°F. 
17. vii. 09. 
103-4 
102-8 
102-6 
3 p.m. Inoculated with 4 c.c. of virulent heart-blood. 
Parasites appeared: very few at film-edge : 3 (PP), 1 (0). 
°/o 
°/o of 
infected. 
r.b.c. 
(P) 
(O) 
(PP) (D) (P 4 ) (PPO) (OO) (0 4 ) (P e ) 
6 
103-0 
10.30 a.m. more parasites 
11 a.m. Treatment: 4-5 
0-1 
2 
40 
47 
2 
9 . . . . 
c.c. Brilliant Green sol. 
3 p.m. ,, ,, ,, ,, 
0-2 
3 
34 
60 
2 
1 ... . 
5 p.m. ,, ,, ,, ,, 
3-0 
3 
45 
38 
2 
2 ... 
102-8 
5.30 p.m. Swelling at 
site of drug-injection. 
7 
103-4 
a.m. yy y, yy yy 
12 m. Swelling lanced. 
0-5 
5 
39 
53 
1 
2 • • • • 
104-0 
5 p.m. 
8 
104-2 
— a.m. 
4-0 
5 
44 
46 
3 
2 • • • • 
9 
104-2 
10.30 a.m. Dog moribund; 
haemoglobinuria, blood 
watery ... 
30-0 
4 
38 
39 
4 
7 14 12 
25. vii. 09. 
11 a.m. Dog killed. The usual appearances of piroplasmosis were 
observed at autopsy. Very little colour was left at the seat of the 
dye injection. 
Control Dog : see under the following experiment. 
1 The abbreviations in this paper are similar to those used in our previous publications. 
(For details of methods used, see p. 161, this volume): 
The signs Stand for corpuscle containing 
(P), (PP), (P 4 ), (P 6 ) ... 1, 2, and 4 or more pyriform parasites respectively. 
(O), (OO) ... ... 1 and 2 rounded parasites respectively. 
(PPO) ... ... 2 pyriform and 1 rounded parasite. 
(D), (DD) ... ... 1 and 2 dividing forms respectively. 
The abbreviation r.b.c. stands for red blood-corpuscles. 
The + signs indicate that parasites were present, but that the percentage of infected 
r.b.c. was too small to determine without counting many thousands of corpuscles. 
