414 
Treatment of Canine Piroplasmosis 
Experiment with Benzopurpurine. 
Bog 2 : Fox-terrier puppy. Weight 9J lbs. 
Received one subcutaneous injection of Benzopurpurine in 1 °/ 0 watery solution, 
48 hours after the parasites first appeared in the dog’s blood. 
Date Day Temp.°F. 
17. Vll. 09. 
25. vii. 09. 9 
—- 3 p.m. Inoculated with 4 c.e. of virulent heart-blood. 
103-2 
103-6 
103-4 
103-0 
7 104-0 
103-6 
103-4 
infected— 
r.b.c. (P) 
1-2 
(O) (PP) (D) (Pi) (PPO) (OO) 
1 48 45 0 
0 0 
a.m. 
a.m. 
a.m. Parasites appeared : very few, 3 (PP) found, 
a.m. Still very few parasites . °/o of % 
at film-edge: 3 (PP), 2 (P 4 ). 
12m. ,, ,, ,, ,, ,, ,, 
12.30 p.m. Treatment : 4-5 
c.c. Benzopurpurine sol. 
3 p.m. . 
5 p.m. 
— a.m. Haemoglobinuria, 
blood watery . 
— a.m. Dog found dead, with the usual appearances due to piro¬ 
plasmosis ; blood watery, etc. Seat of dye injection slightly 
stained. 
1-6 5 26 61 2 
8-0 4 42 43 1 
17. vii. 09. 
25. vii. 09. 
Control Bog 1 . Spaniel puppy. Weight 27 lbs. 
3 p.m. Inoculated with 4 c.c. of virulent heart-blood. 
104-0 
104-8 
104- 4 
103- 6 
105- 0 
104- 2 
a.m. Parasites appeared: only one infected r.b.c. found : 1 (PP). 
a.m. Very few parasites : 4 (PP) found at film-edge. 
a.m. Not examined. 
a.m. One infected r.b.c. per field. 
10 a.m. Bog found dead, with usual appearances of piroplasmosis; 
haemoglobinuria, etc. 
Conclusions regarding the foregoing Experiments with 
Brilliant Green and Benzopurpurine. 
Reference to the percentage counts of the different types of parasites 
encountered in the treated dogs’ corpuscles renders it clear that 
Brilliant Green and Benzopurpurine exert no injurious effect upon the 
parasites and that the piroplasms multiply in the usual manner up to 
the time of the animals’ death. Judging from the few figures contained 
in the protocols it would appear almost as if the injection of the dyes 
increased the percentage of (PP) parasites, thus : 
In Dog 1 the (PP) parasites numbered 47 °/ 0 before, 60 °/ n 4 hours after treatment. 
In Dog 2 „ „ „ „ 45% „ 61 % 24 „ 
