Gr. H. F. Nuttall, 
415 
Experiments with Congo Red. 
Dog 3 : Fox-terrier, aged about 1 year. Weight 14 lbs. 
Received one subcutaneous injection of 4 c.c. of 1 °/ 0 watery solution of Congo Red. 
Date Day Temp.°F. 
16. x. 09. 
31. x. 09. 
1 — Inoculated with 5 c.c. of virulent heart-blood (from Dog 48). 
Q _ 
3 
102-0 
4 
103-0 
5 
105-2 
6 
104-0 
7 
106-0 
10 a.m. Parasites appeared: 3 (O), 4 (PP). 
11.50 
a.m. Treatment with Congo Red. 
8 
103-6 
No parasites found. 
9 
102-6 
9 9 
9 9 
10 
102-6 
9 9 
9 9 
11 
103-2 
99 
9 9 
12 
104-0 
9 9 
9 9 
13 
105-0 
9 9 
9 9 
14 
104-6 
9 9 
,, Dog very weak. 
15 
104-0 
9 9 
99 99 99 
16 
95-0 
a.m. 
No parasites found. Dog very weak. 
7 p.m. Bog found dead. Smears from heart, spleen and liver 
showed no parasites. Death probably due to the drug which 
apparently destroyed both the dog and the parasites. 
Dog 4 : Terrier mongrel, aged about 1- year. Weight 9 lbs. 
Received one subcutaneous injection of 4 c.c. of Congo Red in 0 - 5 °/ 0 watery solution 
48 hours after the parasites appeared in the dog’s blood. 
250 r.b.c. counted for % infected, except on Day 14 when 500 were counted. 
100 parasitized r.b.c. counted for types, except on Day 13 when 50 were counted. 
Date Day Temp.'F. 
26. x. 09. 1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
— Inoculated with 5 c.c. of virulent heart-blood (from Dog 2). 
101-2 
102-0 
101-0 
101- 4 
101-8 
102 - 2 
102-4 Parasites appeared: very few at film-edge: 3 (PP), 1 (O). 
103 0 Few parasites at film-edge : 8 (PP), 6 (O). 
104-8 — a.m. 
12.15 p.m. Treatment 
. °/o° f . 
% 
withCongoRed. Hae- 
r.b.c. (P) 
(O) 
(PP) 
(D) (P 4 ) (OO) (0 3 . 4 ) (PPO") 
moglobinuria 
4-4 • 
54 
42 
2 2 
. 
2.15 p.m. ... 
8-0 • 
55 
38 
1* 3 
3 • 
4.15 p.m. Chromatin 
of parasites tending 
to form one mass ... 
13-6 • 
51 
43 
• 2 
4 • 
* 1 (DD). 
