418 
Treatment of Canine Piroplasmosis 
Control Dog 2 : Fox-terrier, about | year old. Weight lbs. 
Date Day Temp.°F. 
26. x. 09. 1 — Inoculated with 5 c.c. of virulent heart-blood (from Dog 2). 
2 
101-4 
3 
103-0 
4 
101-2 
5 
101-4 
6 
102-6 
7 
103-4 
8 
105-4 
Parasites appeared, few. 
9 
105-2 
,, few. 
10 
103-0 
J J J J 
11 
103-8 
51 5 5 
12 
105-4 
,, more. Haemoglobinuria. 
7. xi. 09. 
13 
103-0 
Bog died of piroplasmosis. 
Control Dog 3 : Collie pup, about § year old. 
Date 
Day Temp.°F. 
26. x. 09. 
1 
— 
Inoculated with 5 c.c. of virulent heart-blood (from Dog 2) 
2 
103-2 
3 
103-8 
4 
103-0 
5 
103-8 
6 
103-6 
7 
102-6 
8 
102-4 
Parasites appeared: only 1 (PP) found. 
9 
102-4 
,, increasing. 
10 
103-0 
,, 3 per field. 
11 
104-8 
,, numerous. Haemoglobinuria. Dog weak. 
6. xi. 09. 
12 
— 
9.15 a.m. Parasites numerous. Bog died of piroplasmosis 
Conclusions regarding the foregoing Experiments 
with Congo Red. 
The three experiments with Congo Red demonstrate very clearly that 
the dye exerts a direct effect upon the parasites. Unfortunately, the 
dye also appears to exert an injurious effect upon the dogs, for all of 
them died. The treated dogs’ blood corpuscles appear to suffer, for 
many of them present a crumpled appearance. 
In the case of Dog 3 the dye-injection was given on the 7th day 
after inoculation, a few parasites having appeared on that day in the 
dog’s blood. No parasites were discoverable in blood-films prepared on 
the 8th to 16th day, and no parasites could be detected in the dog’s 
internal organs at autopsy. 
In the case of Dog 4, the dye-injection was given 48 hours after the 
parasites appeared (10th day after inoculation). The percentage of 
infected corpuscles continued, however, to rise rapidly, so that the 
