G. H. F. Nuttall 
423 
Protocols relating to Test Dogs Nos. I to V which were 
INOCULATED WITH THE BLOOD OF THE RECOVERED DOGS. 
Test Dog I. Collie, about £ year old. Inoculated with blood from the ear-vein of 
Trypanred-treated Dog 1 on the 143rd day after the latter was inoculated. 
Date Day Temp."ft 
19. vii. 09. 
6. viii. 09. 
1 — Inoculated with 10 c.c. of blood from the ear-vein of Dog 1. 
2 103-0 
3 102-6 
4 103 0 
5 104-0 
6 103-8 
7 102-8 
8 103-0 
9 103-0 
10 
106-0 
Parasites appeared: few at i n fectefl 
film-edge. r.b.c. 
°/o 
(P) 
(O) 
(PP) (D) (OO) (PPO) (PO 
11 
106-0 
. 0-5 
2 
27 
64 
1 
2 
1 
3 
12 
106-2 
. 0-4* 
• 
68 
26 
. 
2 
2 
2 
13 
104-2 
. 0-2 
2 
44 
46 
. 
2 
• 
6 
14 
103-0 
. 2-4* 
6 
56 
32 
. 
. 
• 
6 
15 
102-8 
. 0-8 
10 
41 
42 
2 
2 
. 
3 
16 
104 2 
. 1-6 
6 
48 
37 
1 
2 
• 
6 
17 
105-0 
Dog not feeding; looks ill 
(escaping pyriform para¬ 
sites) ... ... ... 5-2 
8 
30 
56 
1 
i 
. 
4 
18 
102-0 
. 20-0 
6 
41 
44 
1 
2 
• 
6 
19 
— 
Dog found dead at 10 a.m. (urine normal in 
bladder). 
* In this case 50 parasites were counted for types and the percentage of infected r.b.c. 
was estimated, 2,000-5,000 r.b.c. being counted by “ fields.” 
Test Dog II. Spaniel, about ^ year old. Inoculated with blood taken from the ear- 
vein of Trypanblue-treated Dog 3 on the 190th day after the latter was inoculated. 
Date Day Temp.°F. 
25. ix. 09. 1 — Inoculated with 10 c.c. of blood from ear-vein of Dog 3. 
2 — 
3 
102-4 
4 
101-6 
5 
101-6 
6 
102-8 
7 
103-2 
8 
104-4 
9 
1034 
10 
104-6 
% 
11 
104-4 
Parasites appeared 4 (PP), 2 (O). r.b.c. (P) (O) 
(PP) 
(D) 
(P«)' 
12 
104-8 
•05 • 
. 
• 
• 
13 
105-8 
9-2* 2 46 
49 
2 
1 
14 
105-0 
4-5 8 28 
57 
• 
7 
15 
104-6 
5-0 9 41 
46 
1 
3 
16 
— 
Dog found dead; haemoglobinuria noted at autopsy. 
* Count only approximate, based on a poorly stained film. 
27—2 
