426 
Treatment of Canine Piroplasmosis 
Conclusions regarding the foregoing experiments. 
It is evident from these experiments that the parasites persist in the 
blood of dogs which have recovered from an acute attack of piroplas¬ 
mosis in consequence of treatment with Trypanblue and Trypanred. 
The blood of such dogs was found to be infective and virulent 143 to 210 
days after the treated dogs were first inoculated. In respect to the 
persistence of the parasites in their blood, the successfully treated dogs 
behave similarly to dogs which have recovered naturally. Two of the 
dogs which recovered after treatment were reinoculated, with negative 
results, on the 213th and 221st day after the primary inoculation which 
produced the acute attack from which they recovered. 
The enumerations of types of parasites in the case of Test Dog Y 
are remarkable in so far as they show an exceptional preponderance of 
(0) parasites over (PP) forms, especially on days 11, 12, 15 and 26. 
In this respect the counts on these days approximate to those observed 
in trypanblue-treated dogs during the period when the (PP) parasites 
are disappearing. In Test Dog Y the (PP) parasites, however, never 
fell below 12—13 °/ 0 . In view of this appearance, coupled with the 
chronicity of the attack and the small percentage of infected r.b.c. 
recorded, it would appear as if some unfavourable condition for the 
multiplication of the parasites existed in this dog. 
4. Confirmatory Experiments upon the Curative Treatment 
of Canine Piroplasmosis by means of Trypanblue. 
Jowett (x. 1909), working on behalf of the Department of Agricul¬ 
ture, Cape Colony, has repeated and fully confirmed the experiments of 
Nuttall and Hadwen upon the cure of canine piroplasmosis in dogs by 
means of Trypanblue. The results of Jowett’s experiments are here 
presented in the form of an abstract. 
To avoid the occasional abscess formation at the seat of the dye 
injection, he recommends intravenous injection of Trypanblue whenever 
possible, this procedure having already been adopted by Nuttall and 
Hadwen in the treatment of cattle with the dye. Jowett’s results may 
be summarized as follows: 
