G-. H. F. Nuttall 
427 
I. Records of Experimental Cases. 
Experiment A. Three dogs were each inoculated with 1 c.c. of 
virulent blood subcutaneously. 
Dog 1. Fox-terrier. Weight 13 lbs. Fever on the afternoon of the 5th day.—Para¬ 
sites appeared 7th day.—On the 8th day 0-75 °/ 0 of r.b.c. infected.—On the 9th day 2-3 °/ 0 
of r.b.c. infected, dog very ill; treatment: 5 c.c. of saturated solution of trypanblue sub¬ 
cutaneously.—On the 10th day, no parasites found in blood-films, 14 hours having elapsed 
since treatment; dog very ill.—11th day, temperature 102-6°—102° F., dog lively.—No 
parasites were observed up to the 30th day the dog making an uninterrupted recovery. 
An abscess at seat of drug-injection healed speedily after being lanced.—On the 36th day 
the dog weighed 15 lbs. ( = 2 lbs. gained). Result: recovery satisfactory and complete. 
No loss of condition. Subsequently reinoculated with negative result. 
Dog 2. Irish terrier (mongrel). Weight 36 lbs. Parasites appeared on the 8tli day ; 
fever.—On the 10th day 0-4 °/ 0 of r.b.c. infected, dog obviously ill; treatment: 10 c.c. sat. 
sol. of trypanblue subcutaneously.—11th day no parasites found.—12th day, temperature 
102-6—102-6° F., dog lively, no parasites.—13th day, painful swelling at site of dye- 
injection, lanced and dressed.—Dog made uninterrupted recovery, no parasites found 
on 14th to 20th, nor on 22nd to 23rd days.—Record ends 30th day. Result: “Recovery 
satisfactory and complete.” 
(Control) Dog 3. Fox-terrier (mongrel). Parasites appeared on the 8th day, 0-5 °/ 0 
of r.b.c. found infected.—9th day dog very ill, haemoglobinuria present; 7 °/o °f r.b.c. 
infected.—10th day r.b.c. mostly infected, dog died of piroplasmosis. 
Experiment B. Two puppies were each inoculated with 1 c.c. of 
virulent blood subcutaneously. (Blood of Dog 1, prior to treatment.) 
Dog 4. Puppy, aged 2 months. Weight 3^ lbs. Parasites appeared on the 8th day. — 
On the 9th day 0-4 °/ 0 of r.b.c. infected; treatment: 3 c.c. of 1 °/ 0 sat. sol. trypanblue sub¬ 
cutaneously.—10th day no parasites.—11th day no parasites, puppy lively.—Recovery 
uninterrupted to 28th day, when record ends. No swelling at site of dye-injection. 
Result: speedy recovery. 
(Control) Dog 5. Puppy, aged 2 months. Weight 4 lbs. Parasites appeared on the 
8th day.—On the 10th day puppy very ill, 20 °/ 0 of r.b.c. infected.— Found dead of piro¬ 
plasmosis on the 11th day. 
Experiment C. Two puppies were each inoculated with 2 c.c. of 
virulent blood subcutaneously. 
Dog 6. Puppy, aged 2 months. Weight 34 lbs. Parasites appeared 5th day, 0‘5 °/ 0 
of r.b.c. found infected.—On 6th day 1 °/ 0 of r.b.c. infected, dog obviously ill; treatment: 
3 c.c. of 1 °/ 0 sat. sol. of trypanblue subcutaneously.—On 7th day temperature 102—102° F., 
no parasites found, dog ill.—8th day, dog better, no parasites.—9th day, apparently 
recovered, no local swelling.—19th—20th day showed uninterrupted recovery without loss 
of condition. Result: Rapid and satisfactory recovery. 
