428 
Treatment of Canine Piroplasmosis 
(Control) Dog 7. Puppy, aged 2 months. Weight 31 lbs. Parasites appeared 6th 
day.—By the 9th day 05 °/ 0 of r.b.e. infected; dog very ill.—10th day 3 to 4 infected r.b.e. 
per field.—12th day no parasites.—From now on to the 19th day temperature varied from 
97° to 102° F., and few or no parasites found.—Puppy became emaciated, jaundiced and 
very anaemic. Died on night of 20th day. 
Experiment D. Conducted on three dogs. 
Dog 8. 6 months old. Weight lbs. Received 2 c.c. bloody serum from pup No. 5 
at death. Intravenous inoculation. Parasites found on the 5th day, dog very dull.—6th 
day dog obviously ill, had haemoglobinuria, 9'5 °/ 0 of r.b.e. infected; treatment: 4-5 c.c. 
sat. sol. of trypanblue subcutaneously.—7th day still very ill, no parasites.—8th day 
temperature 102-2—102° F., no parasites, dog better. 10th—18th day temperature normal. 
Result: “ seems to have made an excellent recovery.” 
Dog 9. Puppy, 2 months old. Weight 4 lbs. Received 2 c.c. of bloody serum from pup 
No. 5 at death. Subcutaneous inoculation. Treated on the third day after inoculation and 
before parasites had appeared. Result: No parasites had appeared by the 16th day. 
Dog lO. Terrier (mongrel). Weight 15J lbs. Treated before inoculation. The dog 
received 15 c.c. of 1 °/ 0 sat. sol. of trypanblue injected subcutaneously. Six days later 
1 c.c. of virulent blood (from Dog No. 8, before treatment) was inoculated intravenously. 
Result: The dog’s temperature was irregular for some days, but parasites could not be 
detected in its blood. This dog is to be tested at a later date to determine if it has been 
rendered immune. 
Experiment E. Conducted on three dogs. 
Dog 11. Pointer puppy, 4 months old. Weight 18| lbs. Received 1 c.c. of blood 
from Dog 8 before the latter was treated. Intravenous inoculation. On the 4th day the dog 
was obviously ill, 13-3 °/o °f r.b.e. found infected; treatment: 1 fluid ounce of sat. sol. of 
trypanblue per os. —5th day, haemoglobinuria, r.b.e. mostly infected; no blue colouration 
of skin or mucous membranes. Result: 6th day dog found dead with typical appearances 
of piroplasmosis. 
Dog 12. Pointer puppy. Weight 19| lbs. Inoculated intravenously (saphenic vein) 
with 1 c.c. of blood from Dog 8 before the latter was treated. Parasites found on 4th day, 
puppy dull.—By the 6th day the dog looked very ill, temperature 105—105° F., more 
parasites present; treatment: 5 c.c. of trypanblue solution injected intravenously.—7th 
day temperature 101-4—102-4° F., dog lively and feeding ; no parasites found. The record 
of this dog ends on the 9th day, the blood examination still negative. Result: dog 
apparently recovering. 
(Control) Dog 13. Puppy, 2 months old. Weight 4 lbs. Inoculated subcutaneously 
with 1 c.c. of blood from Dog 8, before the latter was treated. Parasites found on the 7th 
day.—8th day 1-3 °/ 0 and 9th day 9 °/o of r.b.e. found infected.—10th day dog dying, 
haemoglobinuria present, r.b.e. mostly infected. Result .- dog died with typical appear¬ 
ances of piroplasmosis. 
